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1、醫(yī)學(xué)論文-天然腦活素促細(xì)胞增殖和抗細(xì)胞衰老作用的研究?????????????????作者：李映紅吳正治吳偉康李明張曉麗陳茵楊敏【摘要】?目的：探討天然腦活素對(duì)人胚肺二倍體成纖維細(xì)胞（MRC-5）增殖能力和復(fù)制性衰老的影響及其機(jī)理。方法：光鏡下觀察MRC-5細(xì)胞衰老表型，常規(guī)計(jì)數(shù)細(xì)胞代齡、傳代速度，MTT法檢測(cè)細(xì)胞增殖活度，X-Gal染色法觀察細(xì)胞衰老情況，計(jì)數(shù)衰老細(xì)胞百分率。結(jié)果：天然腦活素可維持細(xì)胞的非衰老表型，增加MRC-5細(xì)胞代齡，天然腦活素孵育MRC-5細(xì)胞的細(xì)胞增殖能力較空白老齡對(duì)照組明顯升
2、高（P＜0.05），細(xì)胞的增殖速度顯著加快。另外，天然腦活素連續(xù)培養(yǎng)的細(xì)胞在老齡階段衰老細(xì)胞數(shù)顯著低于老齡對(duì)照細(xì)胞（P＜0.01）。結(jié)論：天然腦活素可升高M(jìn)RC-5細(xì)胞增殖能力，有效延緩細(xì)胞的復(fù)制性衰老?！娟P(guān)鍵詞】?天然腦活素；人胚肺成纖維細(xì)胞；增殖活度；復(fù)制性衰老；延緩???〔Abstract〕???Objective????To?explore?the?effects?of?Natural?Cerebrolysin?on?cell?proliferation?activity?andreplicat
3、ive?senescence?of?human?embryonic?lung?diploid?fibroblasts?(MRC-5?cells)?and?its?mechanism.?Methods????To?observe?the?changes?of?senescent?phenotype?by?light?microscope,?count?the?passageages?and?velocity,?detect?the?potential?of?cell?proliferation?by?MT
4、T.?The?changes?of?cells?senescenceof?Natural?Cerebrolysin-effected?MRC-5?cells?were?studied?by?X-Gal?staining?method,?and?the?positivecell?rates?of?senescence?cells?were?detected?and?analyzed.．??Results?????Natural?Cerebrolysin?maintainedunaltered?cell?m
5、orphology?and?increased?life?span?of?MRC-5?by?at?least?18-21?passages.?Comparing?withthe?control?senium?cells,?the?proliferation?potential?of?MRC-5?cells?incubated?with?Natural?Cerebrolysinwas?increased?obviously?(P?
6、enhanced?double?of?that?of?the?controlsenium?cells?(P?
7、ells?(P?
8、〕???Natural?Cerebrolysin;?Human?embryonic?lung?diploid?fibroblasts;?Proliferation?activity;?Delay;??Replicative?senescence???衰老（senescence）是細(xì)胞的重要生命現(xiàn)象之一，絕大多數(shù)正常細(xì)胞被認(rèn)為僅有有限的分裂能力，當(dāng)其經(jīng)過(guò)一定的倍增之后，將進(jìn)入終末增殖狀態(tài)，稱(chēng)為細(xì)胞衰老。一般來(lái)講，人類(lèi)胚胎的成纖維細(xì)胞在達(dá)到衰老
