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1、Viruses,immunityandmentaldiseases309Abstract37.AntibodymodulationofcoronavirusencephalitisP.J.Talbot,R.L.Knobler,J.J.BuchmeierScrippsClinicandResearchFoundation,LaJolla,California,U.S.A.(Currentaddress,PJT:InstitutArmand-Frappier,Laval,Quebec,Canada)TheJHMstrainofmurinehepatitisvirus(MHV-4
2、)isusedasananimalmodelofvirus-induc-eddiseasesofthecentralnervoussystem(CNS).Demyelinationresultsfromviralinfect-ionoftheoligodendrocytesbutthisdiseaseisusuallymaskedbyafulminantlethalence-phalomyelitisduetoneuronalinfection.Passivetransferofmonoclonalantibody(MAb)totwoofthreeneutralizatio
3、nsitesontheviralattachmentandfusionglycoproteinE2modu-lateddiseasefromfatalencephalitistodemyelination.Anessentialroleforantibodyalonewasdemonstratedbyprotectionofnudemicefromlethalviralencephalitisafterpas-sivetransferofMAborserumfromMHV-4immuneanimals.Quantitationofantibodyresponseatthee
4、pitopelevelemphasizedtheimportanceofneutralizingantibodylevels.Passivetransferofimmuneserumcontaining0.52ug/mlantibodiesagainstepitopeA(E2),oneofthethreeneutralizationsitesontheE2glycoprotein,wassufficienttoconferprotection,whereasanimmuneserumcontaining0.28pg/mlofsuchantibodiesfailedtopro
5、tectmice.Uponperipheralinoculation,nudemicegeneratedalimitedantibodyresponsetoMHV-4,bothIgGandIgM.Wearecurrentlyinvestigatingwhethersuchalevelofantibodiesissufficienttoconferprotectionagainstasubsequentnormallylethalintracerebralchallen-ge.Thus,appropriateantibodyresponsetoMHV-4isimportant
6、indeterminingtheoutcomeofvirusinfectionoftheCNS.Abstract38.MolecularcharacterizationofmurineretrovirusesinducingspongiformlowermotorneurondiseaseP.Jolicoeur,L.DesGroseillersandE.RassartInstitutdeRecherchesCliniquesdeMontreal,Montreal,CanadaSomeecotropicretrovirusesisolatedfromwildmiceweres
7、howntobeabletoinducehindlimbparalysisintheirnaturalenvironmentorwheninoculatedtonewbornmice.WehaverecentlyclonedtheDNAgenomeofoneofthesevirusesinaprokarioticvectorandshownthatthevirusrecoveredfromthisclonedDNAcouldinduceparalysis.Thisresultindicat-edunambiguou
