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1、TransboundaryandEmergingDiseasesRAPIDCOMMUNICATIONCharacterizationofaNovelChimericSwineEntericCoronavirusfromDiseasedPigsinCentralEasternEuropein2016111211G.J.Belsham,T.B.Rasmussen,P.Normann,P.Vaclavek,B.StrandbygaardandA.B?tner1DTUNationalVeterinaryInstitute
2、,TechnicalUniversityofDenmark,Lindholm,Kalvehave,Denmark2DepartmentofVirology,StateVeterinaryInstituteJihlava,Jihlava,CzechRepublicKeywords:Summaryrecombination;porcineepidemicdiarrhoeavirus;transmissiblegastroenteritisvirusDuringasevereoutbreakofdiarrhoeaand
3、vomitinginapigherdinCentralEast-ernEurope,faecalsamplesweretestedpositiveforporcineepidemicdiarrhoeaCorrespondence:virus(PEDV)andnegativefortransmissiblegastroenteritisvirus(TGEV)usingaA.B?tner.DTUNationalVeterinaryInstitute,commercialRT-qPCRassaythatcandetec
4、tbothofthesecoronaviruses.However,TechnicalUniversityofDenmark,Lindholm,furtheranalyses,usingotherTGEV-andPEDV-speci?cRT-qPCRassays,pro-Kalvehave4771,Denmark.Tel.:+453588videdresultsinconsistentwithinfectionbyeitheroftheseviruses.Sequencingof7858;Fax:+4535887
5、901;E-mail:aneb@vet.anamplicon(ca.1.6kb),generatedbyanRT-PCRspeci?cforthePEDVS-gene,dtu.dkindicatedaveryclosesimilarity(ca.99%identity)torecentlydescribedchimericReceivedforpublicationJune29,2016virusestermedswineentericcoronaviruses(SeCoVs).Theseviruses(with
6、anRNAgenomeofca.28kb)were?rstidenti?edinItalyinsamplesfrom2009butdoi:10.1111/tbed.12579havenotbeendetectedtheresince2012.AcloselyrelatedviruswasdetectedinarchivedsamplesinGermanyfrom2012,buthasnotbeendetectedsubsequently.Buildingontheinitialsequencedata,furth
7、erampliconsweregeneratedandover9kbofsequencecorrespondingtothe30-terminusofthenewSeCoVgenomewasdetermined.SequencecomparisonsshowedthatthethreeknownSeCoVsare≥98%identicalacrossthisregionandcontaintheS-geneand3asequencesfromPEDVwithinabackboneofTGEV,buttheviru
8、sesareclearlydistinctfromeachother.Itisdemonstrated,forthe?rsttime,thatpigsfromwithintheSeCoV-infectedherdseroconvertedagainstPEDVbuttestednegativeinaTGEV-speci?cELISAthatdetectsantibodie
