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《PR基因G886G多態(tài)性與子宮內(nèi)膜異位癥遺傳易感性的關(guān)聯(lián)研究.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在應(yīng)用文檔-天天文庫。
1、·10·婦產(chǎn)與遺傳(電子版)2013年6月第3卷第2期Obstetrics-GynecologyandGenetics，June2013，Vo1．3，No．2DOI：3868／j．issn．2095—1558．2013．02．003·論著·基因G886G多態(tài)性與子宮內(nèi)膜異位癥遺傳易感性的關(guān)聯(lián)研究毛婷宗利麗段紅麗李嘉蔚王玉鳳曾俊趙欣付永貴饒興薔黃郁馨【摘要】目的探討中國南方漢族婦女孕激素受體(progesteronereceptor，PR)基因第8外顯子區(qū)G886G(rs500760)位點(diǎn)單核苷酸多態(tài)性(sin
2、glenucleotidepolymorphism，SNP)與子宮內(nèi)膜異位癥(endometriosis，Eros)遺傳易感性的相關(guān)性。方法收集同期經(jīng)手術(shù)病理證實(shí)的431例Ems患者(Ems組)和499例無Ems的婦女(對(duì)照組)外周血，采用熒光定量PCR為基礎(chǔ)的高分辨率熔解曲線分析(HighResolutionMelting，HRM)技術(shù)檢測P基因G886G位點(diǎn)SNP，通過病例對(duì)照研究評(píng)估SNP和Ems的相關(guān)性。結(jié)果Ems組和對(duì)照組PRG886G位點(diǎn)等位基因A、G分布分別為79．1％、20．9％和79．4％、
3、20．6％，基因型AA、AG、GG分布分別為61．7％、34．8％、3．5％和61．3％、36．1％、2．6％，兩組等位基因及基因型分布差異均無統(tǒng)計(jì)學(xué)意義(P=0．899和0．705)。結(jié)論中國南方漢族婦女PRG886G位點(diǎn)多態(tài)性與Ems遺傳易感性無明顯關(guān)聯(lián)?！娟P(guān)鍵詞】子宮內(nèi)膜異位癥；多態(tài)性，單核苷酸；高分辨率熔解曲線分析StudyontheassociationbetweenthepolymorphismofprogesteronereceptorG886Ggeneandthesusceptibilityt
4、oendometriosisMAO死，ZONGLi—li，DUANHong—li，LIJia—wei，WANGYu一ng，ZENGJun，ZHAOXin，F(xiàn)Yong—gui，RAOXing—qiang，HUANGYu—xin．DepartmentofGynecol—ogyandObstetrics，GuangdongProvinceMaternalandChildHealthHospital，Guangzhou，Guangdong，5lo0loP．R．ChinaCorrespondingauthor：ZONG
5、Li—li，E—mail：zonglili2002@yahoo．tom．en[Abstract]0bjectiveToinvestigatetheassociationofthesinglenucleotidepolymorphism(SNP)ofprogesteronereceptor(PR)geneexon8regionG886G(rs500760)sitewiththegeneticsusceptibilitytoendometriosis(EmsinsouthernHanChinesewomen．Me
6、thodsPeripheralbloodsamplesof431patientswithendometriosis(Emsgroup)and499womenwithnon—endometriosis(controlgroup)werecollected．Allcaseswereconfirmedbyoperationandpathology．ThefluorescentquantitativePCR-basedhighresolutionmelting(HRM)methodwasusedtoevaluatet
7、heSNPofPRG886Gsiteamongallcases．ResultsTheAandGofPRG886Gallelefrequencieswere79．1％．20．9％intheEmsgroupand79．4％，20．6％inthecontrolgroup，respectively．TheAA，AGandGGofPRG886Ggenotypefrequencieswere61．7％，34．8％，3．5％inEmsgroupand61．3％，36．1％．2．6％incontrolgrouprespect
8、ively．Therewerenostatisticallysignificantdifferencesbe—tweenthetwogroups，bothinthePRG886Gallelesdistribution(P=0．899)andgenotypesdistribu—tion(P=0．705)．ConclusionTheremaybenoassociationbetweentheSNPofP
