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《小鼠骨髓間充質(zhì)干細(xì)胞聯(lián)合造血干細(xì)胞治療小鼠肝硬化疾病研究-論文.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在應(yīng)用文檔-天天文庫(kù)。
1、HeilongjiangAnimalScienceandVeterinaryMedicineNo062014小鼠骨髓問充質(zhì)干細(xì)胞聯(lián)合造血干細(xì)胞治療小鼠肝硬化疾病研究李江濤，胡亮，沈吉士，潘琴琴，陸敏，何家梁，孫振國(guó)(1．湖州市農(nóng)業(yè)科學(xué)研究院，浙江湖州313000；2．湖州市吳興區(qū)農(nóng)林發(fā)展局，浙江湖州313000；3．湖州市吳興區(qū)疾病預(yù)防與控制中心，浙江湖州313000；4．浙江金時(shí)代生物技術(shù)有限公司，浙江湖州313000；5．湖州市畜牧獸醫(yī)局，浙江湖州313000)中圖分類號(hào)：$865．1；$865．4
2、文獻(xiàn)標(biāo)識(shí)碼：A文章編號(hào)：1004—7034(2014)06—0030—04關(guān)鍵詞：肝硬化；問充質(zhì)干細(xì)胞；造血干細(xì)胞；小鼠；骨髓摘要：為了驗(yàn)證聯(lián)合應(yīng)用兩種干細(xì)胞治療肝硬化疾病的有效性，試驗(yàn)采用貼壁培養(yǎng)法分離小鼠骨髓間充質(zhì)干細(xì)胞，并在體外單克隆純化，采用免疫磁珠法分離臍帶血造血干細(xì)胞，對(duì)造血干細(xì)胞進(jìn)行無血清培養(yǎng)及生物學(xué)性狀檢測(cè)，然后用間充質(zhì)干細(xì)胞聯(lián)合造血干細(xì)胞附加肝細(xì)胞生長(zhǎng)因子治療Babl／c裸鼠肝硬化。結(jié)果表明：成功純化培養(yǎng)小鼠骨髓問充質(zhì)干細(xì)胞，體外無血清培養(yǎng)獲得臍帶血造血干細(xì)胞：肝細(xì)胞生長(zhǎng)因子40ng／
3、mL、間充質(zhì)干細(xì)胞5×10。個(gè)、造血干細(xì)胞5x10。個(gè)組作用于Babl／c裸鼠肝硬化模型，Babl／c裸鼠肝功能顯著改善，丙氨酸轉(zhuǎn)氨酶由198U／L下降至86U／L，天冬氨酸轉(zhuǎn)氨酶由398．7U／L下降至288．6U／L。說明小鼠骨髓間充質(zhì)干細(xì)胞能夠在體外實(shí)現(xiàn)單克隆純化，小鼠臍帶血造血干細(xì)胞可以在體外無血清培養(yǎng)并大量克隆，干細(xì)胞對(duì)肝硬化疾病具有明顯的治療效果。StudyOnthetreatmentoflivercirrhosisbvthecombination0fmOllsebonemarr0w—der
4、ivedmesenchymalstemcellsandhematopoieticstemcellsinmiceLIJiang—tao，HULiang，SHENJi—shi。，PANQin—qin，LuMin，HEJia—liang，SUNZhen—guo(1．HuzhouAcademyofAgrlculturalSciences，Huzhou313000，China；2．BureauofAcultureandForestryDevelopmentofWuxingDistrict，Huzhou313000
5、，China；3．CenterForDiseaseControlAndPreventionofWuxingDistrict，Huzhou313000，China；4．ZhejiangGeneStemCellBiotechCo．．LTD．Huzhou3l3000，China；5．HuzhouBureauofAnimalHusbandryandVeterinaryMedicine，Huzhou313000，China)Keywords：livercirrhosis；mesenchymalstemcells；
6、hematopoieticstemcells；mouse；bonemarrowAbstract：ToverifytheeffectivenessoftheeombinationofthetwotypesofstemceilsinthetreatmentofmonF~~livercirhosisinBab1／cmice，theattachmentculturemethodwasusedtoisolatenlousebonemarrow—-derivedmesenchymalstemcellsforthei
7、nvitropurificationofmono—clanalantibodies：theimmunomagneticbeadmethodwasusedtoisolatehematopoieticstemcellsfromumbilicalcordbloodfortheculturewithserum—freemediumandthedetectionofbiologicalcharacteristics，andthenthecombinationofmousebonemalTOW—derivedmes
8、enehymalstemcellsandhematopoieticstemcellswithhepatocytegrowthfactorwasappliedinthetreatmentofmouselivercirrhosisinBabl／emice．Theresultsshowedthatthemousebonemarow—derivedmesenchymalstemcellsweresuccessfullypurifiedandcult
