芬維A胺對(duì)人肝癌細(xì)胞株HepG2增殖與細(xì)胞凋亡的影響.pdf

《芬維A胺對(duì)人肝癌細(xì)胞株HepG2增殖與細(xì)胞凋亡的影響.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。

1、海南醫(yī)學(xué)2015年2B第26卷第4期HainanMedJ，F(xiàn)eb．2015，Vo1．26，No．4doi：10．3969／j．issn．1003—6350．2015．04．0173芬維A胺對(duì)人肝癌細(xì)胞株HepG2增殖與細(xì)胞凋亡的影響石勝利，戴小華，馬婭梅，周海華(中山小欖人民醫(yī)院消化內(nèi)科，廣東中山528415)【摘要】目的探討分子靶向藥物芬維A胺(Fenretinide)在體外對(duì)人肝癌細(xì)胞株HepG2的細(xì)胞增殖與細(xì)胞周期的影響。方法體外培養(yǎng)人肝癌HepG2細(xì)胞，實(shí)驗(yàn)組在培養(yǎng)液中加入不同濃度的芬維A胺(2．5lamoFL、5gmol／L、10~tmol／L、20

2、gmol／L)，對(duì)照組培養(yǎng)液中不加芬維A胺，分別孵育48h后，采用MTT法檢測(cè)芬維A胺對(duì)HepG2細(xì)胞增殖的影響，流式細(xì)胞儀分析細(xì)胞周期和凋亡率。結(jié)果芬維A胺能明顯抑制HepG2細(xì)胞增殖，呈時(shí)間和劑量依賴性；芬維A胺處理H印G2細(xì)胞72h，可使Gcq3。期細(xì)胞比例升高，G、S期比例明顯下降，凋亡率明顯上升(P

3、2—O3Efectoffenrefinideontheproliferationandapoptos~ofhumanfivercancereelillnehepG2．SH／Sheng-li,DAIXiao-hua,MAYa-mei,ZHOUHai-hua．DepartmentofGastroenterology,ZhongshanXiaolanPeopleHospital，Zhongshan52841~Guangdong,CHINA【Abstract】0bjectiveToinvestigatethedepressantefectsoffertretinide

4、onhumanlivercancercelllineHepG2cellsvitro．MethodsHumanlivercarcinomalineHepG2cellswereculturedinvitro．TheHepG2cellsofthetestgroupwereincubatedinthemedium、7Iri血fertretinideatdiferentconcentrations(2．5ismoFL，5gmol／L，10~tmol／L，20gmol／L)．nlecellsofthecontrolgroupwereincubatedinthemedium

5、for48hours．MTTmethodwasusedtode．tecttheantiproliferativeratiooffenretinideOllHepG2cells，andfloweytometrywasappliedtoanalyzethecellcycleandapopmficratio．ResultsFertretinidecouldobviouslyinhibittheproliferationofHepG2cells,sho、ngtime—anddose-dependentefects．72hoursafter也eHepG2cellswer

6、etreatedwithfenretinide，thepercentageofHepG2cellsinG0~GIperiodincreased，andthatinG2~MperiodandSperioddecreased，withtheaopototicratioofHepG2cellsin-creasedsignificantly(P<0．05)．ConclusionFeuretinidecaninhibitproliferationandpromoteapoptosisofhumanlivercancercelllineHepG2cellsinvitro．

7、【Keywords】Fenretinide；HumanlivercancercelllineHepG2；Cellproliferation；Apoptosis基金項(xiàng)目：2012中山市科技局立項(xiàng)課題(編號(hào)：20122A106)通訊作者：石勝利。E—mail：shishengli2004@163．cQm【16】WySSJM，AboukarshN，OparilS．Sensorydenervationofthekid—HTN—2Tria1)：arandomisedcontrolledtrial[J】．Lancet，2010，neyattenuatesrenovascu

8、larhypertensioninth