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1、昆明醫(yī)科大學(xué)學(xué)報(bào)2015，36(5)：5～7CN53—1221／RJournalofKunmingMedicalUniversity舌鱗癌Cal27裸鼠移植瘤模型的建立及其意義吳京蔓，吳勇(昆明醫(yī)科大學(xué)附屬12腔醫(yī)院口腔頜面外科，云南昆明650031)[摘要]目的為抑癌藥物的臨床研究建立人舌鱗癌動(dòng)物模型．通過模擬與人體內(nèi)環(huán)境及生物學(xué)特性相似的動(dòng)物模型，觀察腫瘤細(xì)胞的生物學(xué)、病理學(xué)行為及轉(zhuǎn)移情況．方法選4～6周BALB／C—nu裸鼠，將體外培養(yǎng)的人舌鱗癌Ca127細(xì)胞接種于裸鼠右腋皮下，觀察裸鼠體質(zhì)量、皮下成瘤情況、腫瘤體積；待裸鼠體質(zhì)量下降至原體質(zhì)量的25％時(shí)，用頸椎脫臼法處死裸鼠
2、，剝離瘤體組織進(jìn)行解刨學(xué)觀察及病理學(xué)觀察．結(jié)果實(shí)驗(yàn)裸鼠在接種Ca127細(xì)胞第7～12d后右腋下出現(xiàn)腫瘤，接種濃度為每只0．1mL約2X106個(gè)細(xì)胞，成瘤率為86．7％．HE染色示腫瘤組織呈典型鱗癌表現(xiàn)；淋巴結(jié)及主要臟器未見轉(zhuǎn)移．結(jié)論成功建立舌鱗癌Ca127裸鼠移植瘤模型．此動(dòng)物模型能較客觀地反映人舌鱗癌的生物學(xué)行為，為進(jìn)行抑癌藥物的臨床研究與治療打下良好基礎(chǔ)．[關(guān)鍵詞]舌鱗癌；Ca127細(xì)胞；裸鼠；移植瘤；動(dòng)物模型[中圖分類號(hào)]R739．8[文獻(xiàn)標(biāo)識(shí)碼]A[文章編號(hào)]2095-610X(2015)05—00O5—04CarcinomaCal27CellsMOdelinNUdeMic
3、eWUJing-man．WUYong(Dept．ofOralandMaxillofacialSurgery，AfiliatedStomatologicalHospitalofKunmingMedicalUniversity，KunmingYunnan650031，China)[Abstract]ObjectiveToestablishananimalmodelofhumantonguesquamouscellcarcinomaforclinicalresearchofanticancerdrugs，Thebiologicalandpathologicaltumorcellbehav
4、iorandmetastasiswereobservedbyanimalmodelwithenvironmentalandbiologicalcharacteristicssimilartohumanbody．MethodsFirst，theselected4—6week—oldBALB／C—HanudemiceweresubjectedtosubcutaneousinoculationinrightarmpitswiththeculturedtonguesquamouscellcarcinomaCa127cells．Then，themiceweight，conditionoftu
5、morgrowth，lengthandwidthoftumorwerecheckedandrecorded．Finally．themicewerekilledwitheervicaldislocationmethodwhentheirweightwaslessthan25％ofinitialweight．Andthetumortissueswerepeeled0fff0ranatomicalandpathologicalobservation．ResultsThetumorsappearedinrightarmpits7—12daysafterinoculationinnudemi
6、ce．Eachmousewasinoculatedwithadoseof0．1ml，whichcontained2×10Cal27cells，tumorformationratewas86．7％．HEstainingoftumortissueshowedthatsquamouscellperformancewastypical，therewerenometastasisfoundforlymphnodeandmainorgans．ConclusionsAnimalmodelsweresuccessfullyestablishedwithtonguesquamouscellcarci
7、nomaCa127cellsinnudemice．Themodelcanreflectrelativelythebiologicalbehaviorofhumantonguesquamouscellcarcinoma，whichlaysagoodfoundationfortheclinicaltreatmentandresearchofanticancerdrugs．[Keywords]Tonguesquamouscellcarcinoma；Ca127cells；Nu
