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《Smac小分子compound3調(diào)節(jié)順鉑誘導(dǎo)卵巢癌細(xì)胞SKOV3凋亡的研究.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫(kù)。
1、第5O卷第6期山東大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)2012年6月VO1．50NO．6JOURNALOFSHANDONGUNIVERSITY(HEALTHSCIENCES)Jun．2012文章編號(hào)：1671—7554(2012)06—0061—04DOI：10．6040／j．issn．1671-7554．2012．06．013Smac小分子compound3調(diào)節(jié)順鉑誘導(dǎo)卵巢癌細(xì)胞SKOV3凋亡的研究喬真，劉培淑，韓冰，王毓(山東大學(xué)1．醫(yī)學(xué)院，濟(jì)南250012；2．齊魯醫(yī)院婦產(chǎn)科，濟(jì)南250012)摘要：目的探討Smac小分子compound3調(diào)節(jié)順鉑對(duì)卵巢癌細(xì)胞SK
2、OV3凋亡及生長(zhǎng)抑制作用。方法噻唑藍(lán)法檢測(cè)Smac小分子compound3單獨(dú)應(yīng)用組、順鉑單獨(dú)應(yīng)用組及二者聯(lián)合應(yīng)用組對(duì)卵巢癌細(xì)胞SKOV3的生長(zhǎng)抑制率、存活率的影響；并繪制生長(zhǎng)曲線。AnnexinV／PI雙標(biāo)流式細(xì)胞術(shù)檢測(cè)Smac小分子compound3單獨(dú)應(yīng)用組、順鉑單獨(dú)應(yīng)用組及二者聯(lián)合應(yīng)用組對(duì)SKOV3凋亡率的影響。Western印跡檢測(cè)Smac小分子compound3單獨(dú)應(yīng)用組、順鉑單獨(dú)應(yīng)用組及二者聯(lián)合應(yīng)用組對(duì)SKOV3的x連鎖凋亡抑制蛋白(X—linkedinhibitorofapoptosisprotein，XIAP)、半胱氨酸一天冬氨酸蛋白
3、酶一3(caspase一3)的表達(dá)影響。結(jié)果Smac小分子compound3單獨(dú)應(yīng)用組、順鉑單獨(dú)應(yīng)用組及二者聯(lián)合應(yīng)用組12、24、48hSKOV3生長(zhǎng)受到抑制，且呈時(shí)間依賴性。二者聯(lián)合應(yīng)用組與順鉑單獨(dú)應(yīng)用組比較生長(zhǎng)抑制率明顯升高。二者聯(lián)合應(yīng)用組的凋亡率高于順鉑單獨(dú)應(yīng)用組及Smac小分子compound3單獨(dú)應(yīng)用組。Smac小分子compound3單獨(dú)應(yīng)用組及二者聯(lián)合應(yīng)用組SKOV3的XIAP表達(dá)降低，二者聯(lián)合應(yīng)用組的caspase一3的激活片段表達(dá)明顯高于其他兩組。結(jié)論Smac小分子compound3能夠調(diào)節(jié)順鉑對(duì)SKOV3生長(zhǎng)抑制作用，促進(jìn)順鉑對(duì)卵巢
4、癌細(xì)胞SKOV3凋亡作用。Smac小分子compound3通過(guò)影響XIAP的表達(dá)來(lái)實(shí)現(xiàn)其促凋亡及生長(zhǎng)抑制作用。關(guān)鍵詞：卵巢腫瘤；Smac小分子compound3；SKOV3細(xì)胞；細(xì)胞凋亡；順鉑中圖分類號(hào)：R711．75文獻(xiàn)標(biāo)志碼：ASmacmimicpotentiatescisplatin-inducedcellapoptosisofovariancancercellsQIAOZhen，LIUPei—shu，HANBing，WANGYu(1．ThemedicalschoolofShandongUniversity，Jinan250012，China；2
5、．DepartmentofobstetricsandgynecologyofQiluHospital，Jinan250012，China)Abstract：OMeetiveToinvestigatetheeffectofasmallSmacmimicmolecule(compound3)oncisplatin—inducedcellapoptosisandgrowthinhibitionofovariancancercelllines．MethodsCellviabilityandthegrowthinhibitionratiooftheovarian
6、cancercelllinesSKOV3weremeasuredbyMTTinthecompound3-treatedgroup，thecisplatin—treatedgroupandthecombinationtreatmentgroup．ApoptosisandproteinexpressionweredetectedbyflowcytometryandWesternblot．ResultsCellviabilitywasinhibitedinthethreegroups，anditwastime—dependent．Thegrowthinhib
7、i—tionratioofSKOV3inthecombinationtreatmentgroupwasmoresignificantlyincreasedthanthecisplatin—treatedgroup，andtheapoptoticrateofSKOV3inthecombinationtreatmentgroupwasmoresignificantlyincreasedthanintheothergroups．ExpressionsofXIAPinSKOV3ceUsweredecreasedinthecompound3-treatedgro
8、upandthecombina-tiontreatmentgroup．Activatedfra
