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《產(chǎn)漆酶裂褶菌篩選和其產(chǎn)酶培養(yǎng)條件優(yōu)化探究》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、產(chǎn)漆酶裂褶菌篩選和其產(chǎn)酶培養(yǎng)條件優(yōu)化探究 摘要：從采自秦嶺的森林腐木菌苔上分離到一株產(chǎn)漆酶的裂褶菌（SchizophyllumcommuneFr.）菌株mys005，對其產(chǎn)漆酶培養(yǎng)方式、培養(yǎng)基組成及培養(yǎng)條件進(jìn)行了優(yōu)化試驗(yàn)。結(jié)果表明，裂褶菌菌株mys005的產(chǎn)漆酶適宜培養(yǎng)方式為液體淺層振蕩培養(yǎng)，含有Cu2+、Mn2+、Zn2+的微量元素復(fù)合液、洋蔥（AlliumcepaL.）、麥（TriticumaestivumL.）麩對其產(chǎn)漆酶都有較大的促進(jìn)作用，而愈創(chuàng)木酚則對其生長和產(chǎn)漆酶有抑制作用。由新鮮馬鈴薯（SolanumtuberosumL.）200g、K2HPO4
2、1.00g、NaCl0.50g、MgSO4·7H2O0.50g、NaNO32.50g、CaCl2·2H2O0.10g、FeCl30.02g、吐溫-801.20mL溶于1000mL去離子水中組成的培養(yǎng)基在添加微量元素復(fù)合液（50mg/mLCuCl2·2H2O+20mg/mLMnSO4·H2O+10mg/mLZnSO4·7H2O）3mL、洋蔥100g、麥麩10g后，并且培養(yǎng)基起始pH4.5、培養(yǎng)溫度30℃、培養(yǎng)搖床轉(zhuǎn)速200r/min、培養(yǎng)時(shí)間7d，則搖瓶發(fā)酵的漆酶產(chǎn)酶量最高可達(dá)1121U/mL，顯示出了良好的工業(yè)開發(fā)潛力。關(guān)鍵詞：漆酶；裂褶菌（Schizophyl
3、lumcommuneFr.）；篩選；產(chǎn)酶培養(yǎng)基；優(yōu)化20中圖分類號(hào)：Q554+.9；S646.1+9；Q93-33文獻(xiàn)標(biāo)識(shí)碼：A文章編號(hào)：0439-8114（2013）12-2792-06IsolationofSchizophyllumcommuneProducingLaccaseandOptimizationofLaccaseProductionConditionsZHAOWen-juan，XUSheng-yun，RENPing（EnzymeEngineeringInstituteofShaanxiSciences/ShaanxiEngineeringCent
4、erforEnzymeTechnology，Xi’an710600，China）Abstract：ASchizophyllumcommuneFr.strainmys005producinglaccasewasisolatedfromtherottenwoodlawninforestofQinlin.Theculturemethodforlaccaseproduction，mediumcompositionandcultivationconditionswasoptimized.Resultsshowedthatthesuitablecultivationmeth
5、odforS.communestrainmys005toproducelaccasewasshallowliquidshakingculture.TraceelementscompoundliquidcontainingCu2+，Mn2+，Zn2+，onion（AlliumcepaL.），wheat（TriticumaestivumL.）brancouldpromotelaccaseproduction；whileguaiacolcouldinhibitthegrowthandlaccaseproduction.Ifaddingtraceelementcompo
6、undsolution（50mg/mL20CuCl2·2H2O+20mg/mLMnSO4·H2O+10mg/mLZnSO4·7H2O）3mL，onion100g，wheatbran10g，inthemediumcontainingfreshpotato（SolanumtuberosumL.）200g，K2HPO41.00g，NaCl0.50g，MgSO4·7H2O0.50g，NaNO32.50g，CaCl2·2H2O0.10g、FeCl30.02g、tween-801.20mLin1000mLdeionizedwater，andkeepingthestartin
7、gpHat4.5，startingtemperatureat30℃，cultureshakerspeedat200r/min，culturingfor7d，thelaccaseenzymeproductionyieldinshakeflaskfermentationcouldbeuptoamaximumof1121U/mL，showinggreatpotentialforindustrialdevelopment.Keywords：laccase；SchizophyllumcommuneFr.；isolation；enzymeproducingmedium；op
8、timization漆酶
