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1、纖維素酶產(chǎn)生菌的篩選_鑒定和產(chǎn)酶條件優(yōu)化30纖維素酶產(chǎn)生菌的篩選_鑒定和產(chǎn)酶條件優(yōu)化30纖維素酶產(chǎn)生菌的篩選_鑒定和產(chǎn)酶條件優(yōu)化30纖維素酶產(chǎn)生菌的篩選_鑒定和產(chǎn)酶條件優(yōu)化30纖維素酶產(chǎn)生菌的篩選_鑒定和產(chǎn)酶條件優(yōu)化30纖維素酶產(chǎn)生菌的篩選_鑒定和產(chǎn)酶條件優(yōu)化30纖維素酶產(chǎn)生菌的篩選_鑒定和產(chǎn)酶條件優(yōu)化30纖維素酶產(chǎn)生菌的篩選_鑒定和產(chǎn)酶條件優(yōu)化30纖維素酶產(chǎn)生菌的篩選_鑒定和產(chǎn)酶條件優(yōu)化30纖維素酶產(chǎn)生菌的篩選_鑒定和產(chǎn)酶條件優(yōu)化30纖維素酶產(chǎn)生菌的篩選_鑒定和產(chǎn)酶條件優(yōu)化30纖維素酶產(chǎn)生菌的篩選_鑒定和產(chǎn)酶條件優(yōu)化30纖維素酶產(chǎn)生菌的篩選
2、_鑒定和產(chǎn)酶條件優(yōu)化30纖維素酶產(chǎn)生菌的篩選_鑒定和產(chǎn)酶條件優(yōu)化30纖維素酶產(chǎn)生菌的篩選_鑒定和產(chǎn)酶條件優(yōu)化30纖維素酶產(chǎn)生菌的篩選_鑒定和產(chǎn)酶條件優(yōu)化30纖維素酶產(chǎn)生菌的篩選_鑒定和產(chǎn)酶條件優(yōu)化30第50卷第5期第年5期20113月湖北農(nóng)業(yè)科學(xué)Vol．50No.5Mar．，2011HubeiAgriculturalSciences纖維素酶產(chǎn)生菌的篩選、鑒定和產(chǎn)酶條件優(yōu)化阮周波，王憶麗，何未男，鄭江燕，張霞，陳瓊，胡松英，張應(yīng)烙321004）（浙江師范大學(xué)化學(xué)與生命科學(xué)學(xué)院，浙江金華摘要：采用稀釋平板法分離馬鈴薯瓢蟲腸道菌，利用剛果紅平板法對(duì)
3、產(chǎn)纖維素酶菌株進(jìn)行初篩，選取透明圈較大的菌株進(jìn)行搖瓶發(fā)酵復(fù)篩，根據(jù)菌株形態(tài)、生理生化特征對(duì)菌株進(jìn)行初步鑒定，通過正交法優(yōu)化產(chǎn)酶條件。結(jié)果表明，經(jīng)初篩和復(fù)篩得到1株酶活相對(duì)較高的菌株B－12，經(jīng)初步鑒定為芽孢桿菌屬（Bacillussp．）。1．25％麥芽浸粉為碳源、1．5％KNO3為氮源、0．2％的NaCl、0．1％的CMC－Na、接種量6％、培養(yǎng)時(shí)間44h為B-12產(chǎn)酶的適宜條件。優(yōu)化后發(fā)酵液中的內(nèi)切葡聚糖酶活（CMCA）為111．710U／mL，較培養(yǎng)44h后的酶活提高了8.78％；濾紙酶活（FPA）為35．017U／mL，提高了387．
4、23％；β－葡萄糖苷酶酶活（BGL）為116．799U／mL，提高了700．38％。關(guān)鍵詞：馬鈴薯瓢蟲；腸道菌；纖維素酶；優(yōu)化中圖分類號(hào)：Q935文獻(xiàn)標(biāo)識(shí)碼：A文章編號(hào)：0439－8114（2011）05－0915-06Screening，IdentificationandCulturalConditionOptimizationofCellulase-producingStrainsfromtheIntestineofHenosepilachnavigintioctomaculataRUANZhou－bo，WANGY30i－li，HEWei
5、－nan，ZHENGJiang－yan，ZHANGXia，CHENQiong，HUSong－ying，ZHANGYing－lao（CollegeofChemistryandLifeSciences，ZhejiangNormalUniversity，Jinhua321004，Zhejiang，China）Abstract：ThebacteriafromtheintestineofHenosepilachnavigintioctomaculatawereisolatedthroughthepourplatemethod．Theywerepreli
6、minarilyscreenedusingCongoredmediumplatemethod，andthenscreenedaccordingtotheircellulaseactiv-itiesbyflaskshakingfermentationmethod．Thestrainwasidentifiedbasedonmorphologicalandphysiologicalcharacters．Inaddition，theculturesubstratesandfermentationconditionswereoptimizedbyort
7、hogonalexperimentmethod．Ahighcellu-lase－producingstrainB－12wasscreenedfromtheintestineofH．vigintioctomaculataanditwasidentifiedasBacillussp．．Thebestcultureconditionswereasfollows：substratewere1．25％maltmeal，1．5％KNO3，0．2％NaCl，and0．1％CMC－Na，inoculationquantitywas6％，culturetime
8、was44h．Inthiscondition，theenzymeactivityofCMCase，filterpaperenzyme（FPA），andβ－gluco
