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1、江蘇省2012年柯薩奇病毒A組16型基因特征分析單軍,嵇紅,包林,付建光,王慎驕,湯奮揚(yáng),周明浩,朱葉飛*(江蘇省疾病預(yù)防控制中心急性傳染病防制所,江蘇南京21009)[摘要]目的:了解江蘇省2012年柯薩奇病毒A組16型(CA16)分離株VP1區(qū)基因特征。方法:選取江蘇省2012年14株CA16病毒分離株,用1對(duì)特異性引物進(jìn)行VP1區(qū)核苷酸序列擴(kuò)增,對(duì)擴(kuò)增產(chǎn)物進(jìn)行測(cè)序,利用生物信息學(xué)軟件對(duì)序列進(jìn)行分析,結(jié)果與CA16參考株序列進(jìn)行同源性比較并構(gòu)建基因進(jìn)化樹。結(jié)果:14株CA16分離株VP1區(qū)核苷酸和氨基酸同源性分別為90.5%~100%和98.7%~100%,與國(guó)際C
2、A16標(biāo)準(zhǔn)株G10的核苷酸和氨基酸同源性分別為75.5%~76.7%和91.6%~92.3%。江蘇省2012年的CA16分離株全部屬于B1基因亞型,同時(shí)包含B1a和B1b兩條進(jìn)化分支。結(jié)論:江蘇省2012年有CA16病毒的B1a與B1b兩種進(jìn)化分支共同存在與循環(huán),且呈現(xiàn)以B1b基因亞型為優(yōu)勢(shì)型別、B1a基因亞型為輔助型別的傳播模式;無論是優(yōu)勢(shì)型別還是輔助型別,均各自呈現(xiàn)密切的親緣關(guān)系。[關(guān)鍵詞]手足口病;柯薩奇病毒A組16型;VP1基因;基因型[中圖分類號(hào)]R373.2[文獻(xiàn)標(biāo)識(shí)碼]AAnalysisongeneticcharacterofCoxsackievirusA
3、16strainsisolatedinJiangsuProvincein2012SHANJun,GIHong,BAOLin,FUJian-guang,WANGShen-jiao,TANGFen-yang,ZHOUMing-hao,ZHUYe-fei*(DepartmentofAcuteInfectiousDiseaseControlandPrevention,JiangsuProvincialCenterforDiseasePreventionandControl,Nanjing210009,China)[Abstract]Objective:Toanalyzetheg
4、eneticcharacteristicofCoxsackievirusA16(CA16)strainsisolatedfromJiangsuProvincein2012.Methods:14CA16strainsisolatedfromthesamplesofhand-foot-mouthdisease(HFMD)patientswereselectedtoconductVP1geneamplificationusingspecificprimers,andthensequenced.Thehomologyandphylogeneticanalyseswerecond
5、uctedbetweenthe14isolatesandotherCA16strainsdownloadedfromGenbank,basedonthesequenceofVP1gene.Results:ThesequenceanalysisshowedthatthehomogeneityofthenucleotideandaminoacidofVP1genewere90.5%~100%and98.7%~100%,respectivelyamong14isolatedCA16strains.ThenucleotideandaminoacidhomologiesofVP1
6、genewere75.5%~76.7%and91.6%~92.3%,respectivelyamongtheprototype(G10)andJiangsuCA16strains.Allofthe14CA16isolatedstrainsfellwithingenogroupBandtheyweredividedinto2branches:B1aandB1b.Conclusions:ThetransmissionmodewasshowedtobeonedominantsubgenotypeofB1bandonesecondarysubgenotypeofB1aco-ci
7、rculatedtogether.Bothtwosubgenotypesshowedclosephylogeneticrelationshipintheirownsubgenotyperespectively.[KeyWords]Hand-foot-mouthdisease;CoxackievirusA16;VP1gene;Genotype[基金項(xiàng)目]江蘇省臨床醫(yī)學(xué)中心(高技術(shù)平臺(tái))(ZX201109)*通訊作者,E-mail:jszyf@jscdc.cn;單軍和嵇紅為并列一作手足口?。╤and-foot-mouthdisease,HFM