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《沙門菌pcr檢測方法的建立與其初步應(yīng)用》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、揚卅I大學(xué)碩士學(xué)位論文4results．ItwasshownthatthePCRassaycoulddetectartificialSalmonellacontaminatedsamples，andthedetectionlimitwas43．85pgDNAor1CFUaftercultivation．TheseresultssuggestedthataspecificandsensitivePCRmethodWassuccessfullyestablished，andthisnewmethodprovidedausefulwayforthedetectionandmonit
2、oringofSalmonella．2．PreliminaryapplicationofthePCRassayforthedifferenttypesofsamplesFromJuly2008toJanuary2009，1082samplesofanusswab，liverandeggsfromavian，466samplesofanUSmop，meatandlymphnodefromswine，58watersamplesfrompoolandenvironmentinYangzhouregionweredetectedbythePCRassay,andSalmonella
3、Wasisolatedfromthepositivesamples．Resultsshowedthat：therewere41positivesoutofaviansamples，15positivesofswinesamples，2positivesoutofwatersamples．Wetotallygained58isolatesofSalmonella．ThePCRmethodWasverifiedbyabundancesamples，whichindicatedthatitWassuitablefordetectionofsamplesfromavian，swine
4、，water,etc，andapplicablefordifferenttypesofsampletestssuchastheanUSmop，liver,watersamples，meat．3．AntibioticresistanceofSalmonellaisolates100salmonellaisolatesweretestedfortheirsensitivityto18antimicrobialagentsbythediskdiffusionmethodrecommendedbyClinicalandLaboratoryStandardInstitutein2007
5、．TheresultsshowedthatthelevelofantibioticresistanceofSalmonellaishigh，andthesituationofmultipleantibioticresistanceofSalmonellaisnotoptimistic．Although100salomnellaisolateswerehi曲lysensitivetocephalosporins，theywerestronglyresistanttonalidixicacid，tetracyclineandstreptomycin．Studiesondrug-r
6、esistantspectrumshowedthatthemulti-drugresistanceof100isolatesrangingfrom2resistanceto13resistance，mainlyconcentratedinthe2resistanceto5resistanceand13resistance，whichaccountedfor78．13％．Thesedatashowedthatinourcountry,thephenomenonofmulti-drugresistanceofSalmonellaisveryserious．Theisolatesw
7、erehighlyresistanttoabroadspectrumofdrugsinourstudy,especiallyfourSalmonellaisolatesresistanceto13kindsofantibiotics．Inthisstudy,isolatesfromdifferentsourcesshoweddifferentsensitivityleveltodifferenttypesofantimicrobial．Therefore，itisimportanttochooserat
