cik對(duì)人肺腺癌耐順鉑細(xì)胞a549ddp殺傷效應(yīng)及逆轉(zhuǎn)耐藥的初步研究

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1、遵義醫(yī)學(xué)院碩士學(xué)位論文CIK對(duì)人肺腺癌耐順鉑細(xì)胞A549/DDP殺傷效應(yīng)及逆轉(zhuǎn)耐藥的初步研究姓名：李應(yīng)林申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：內(nèi)科學(xué)指導(dǎo)教師：李寧201205遵義醫(yī)學(xué)院碩士學(xué)位論文CIK對(duì)人肺腺癌耐順鉑細(xì)胞(A549／DDP)殺傷效應(yīng)及逆轉(zhuǎn)耐藥的初步研究中文摘要目的：探討CIK細(xì)胞對(duì)人肺腺癌耐順鉑細(xì)胞A549／DDP體外殺傷效應(yīng)及逆轉(zhuǎn)作用。方法：1．CIK細(xì)胞的制備：取健康人外周血單個(gè)核細(xì)胞，經(jīng)IFN．Y，IL．2，抗CD3單抗等聯(lián)合誘導(dǎo)CIK細(xì)胞。2．用MTT法分別檢測(cè)順鉑、CIK細(xì)胞、CIK聯(lián)合順鉑對(duì)人肺腺癌細(xì)胞

2、A549及人肺腺癌耐順鉑細(xì)胞A549／DDP的殺傷活性，計(jì)算耐藥倍數(shù)，逆轉(zhuǎn)倍數(shù)。結(jié)果：1、順鉑對(duì)人肺腺癌細(xì)胞A549及人肺腺癌耐順鉑細(xì)胞A549／DDP的50％殺傷濃度(IC50)值分別為1．823pg／ml、6．392／衛(wèi)g／ml，差異有統(tǒng)計(jì)學(xué)意義(尸

3、)為7．955×105個(gè)細(xì)胞／ml，差異無(wú)統(tǒng)計(jì)學(xué)意義(P>D．∞)。3、通過(guò)實(shí)驗(yàn)確定CIK細(xì)胞對(duì)人肺腺癌耐順鉑細(xì)胞A549／DDPl0％殺傷密度(IClo=4．130×105個(gè)細(xì)胞／nil)為CIK細(xì)胞作用人肺腺癌耐順鉑細(xì)胞A549／DDP的無(wú)毒劑量，以排除CIK細(xì)胞對(duì)人肺腺癌耐順鉑細(xì)胞A549／DDP的干擾，選定CIK細(xì)胞IClo值以下的三個(gè)密度(1×105個(gè)細(xì)胞／ml、2×105個(gè)細(xì)胞／ml、3×105個(gè)細(xì)胞／m1)先與人肺腺癌耐順鉑細(xì)胞A549／DDP作用44小時(shí)，再加順鉑作用4小時(shí)后，其結(jié)果IC50值分別降至1

4、．7431,tg／ml、1．336pg／ml、0．8831．tg／ml，差異有統(tǒng)計(jì)學(xué)意義(P

5、鉑對(duì)人肺腺癌耐順鉑細(xì)胞A549／DDP殺傷活性。逆轉(zhuǎn)倍數(shù)隨著CIK密度增加而增大，CIK細(xì)胞能部分逆轉(zhuǎn)人肺腺癌耐順鉑細(xì)胞A549／DDP對(duì)順鉑的耐藥，其機(jī)制有待進(jìn)一步研究證實(shí)。關(guān)鍵詞：CIK，肺癌；肺腺癌細(xì)胞；耐藥．遵義醫(yī)學(xué)院碩士學(xué)位論文PreliminarystudyofthereversaleffectsandtheinhibitioneffectsofCIKonhumancisplatinresistantcelllineA549／DDPAbstract：Objective：Toinvestigatetherev

6、ersaleffectsandtheinhibitioneffectsofCⅡ(onhumancisplatinresistantcelllineA549／DDPinvitro．Methods：1．ThepreparationofCIK：PBMCcellswerecollectedfrom20mlbloodofhealthydonoLitwasinducedbyIF-y，IL-2，CD3McAbandSOontoobtainCIKcells．2．Todetectthecytotoxicityofcisplatin，CI

7、KcellsandtheconbinationofthetwoabovebyMTTassay．Caculatetheresistentindexandthereversalfold．Results：1．TheIC50(50％inhibitingconcentration)valuewas1．8231xg／mland6．392pg／mlwhenA549andA549／DDPcellsweretreatedwithcisplatin．thetwogroupssignificantlydifferentfromcontrol

8、s但0．05)．TheIC50valuewas7．65