資源描述：
《igf-1對大鼠局灶性腦缺血再灌注后vegf、bfgf表達的影響與意義》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。
1、中文摘要間點bFGFmRNA表達均較同時間點高，P<0．05，有統(tǒng)計學意義。7、HE染色，光鏡下觀察，假手術(shù)組腦組織結(jié)構(gòu)無明顯異常。缺血再灌注24h正常神經(jīng)細胞數(shù)減少明顯，神經(jīng)元空泡變性多，可見細胞核固縮，組織疏松，細胞間隙增大。IGF。1治療組腦組織損傷程度較輕，皮層神經(jīng)元細胞空泡變性較少，間質(zhì)水腫輕，正常細胞數(shù)增多。結(jié)論：l、本實驗通過側(cè)腦室注入外源性IGF．1，明顯改善了大鼠腦缺血再灌注后的神經(jīng)功能，證明IGF．1對缺血再灌注損傷有保護作用。2、腦缺血后可誘導VEGF、bFGF基因及蛋白表達增高，是自我保護機制的一種反應。3、IGF．1可以使腦缺血再灌注后腦組織中VEGF、bFGF的
2、表達進一步增高。4、IGF．1對腦缺血的保護作用可能是通過增加腦組織中VEGF、bFGF基因及蛋白表達而實現(xiàn)的。5、本實驗探討了IGF．1對腦缺血的保護作用及可能機制，為臨床應用IGF．1治療腦缺血損傷提供了理論依據(jù)。關(guān)鍵詞：缺血再灌注；胰島素樣生長因子．1；血管內(nèi)皮生長因子；堿性成纖維生長因子；基因表達英文摘要TheeffectandsignificanceoflGF一1ontheexpressionofVEGFandbFGFaftercerebrallSCilemlcreperlusloninrats●_●n■●ABSTRACTObjective：Ischemiccerebrovasc
3、ulardiseaseiSthecommoncentralnervoussystemdiseaseanditsrateofmortalityanddisabilityisveryhigh．Ischemiccerebrovasculardisease，heartdiseaseandmalignanttumorarethethreediseasesleadingtodisabilityinmostcountriesandseverelythreatenhumans’healthy．Howtoimprovethecerebralbloodflowoftheischemicbraindamagea
4、ndpromotetherepairandregenerationofthedamagedneuronhavebecomethefocusanddifficultyofmanystudies．Therefore，researchonthenervegrowthfactorsandvasculargrowthfactorscausedgreatextraattention．Currently,insulin—likegrowthfactor-1(IGF一1)、vascularendothelialgrowthfactor(VEGF)andbasicfibroblastgrowthfactor
5、(bFGF)arethemostlypopularstudiedfactors．Alongwiththecontinuousdeepeningofthestudy,endogenousIGF一1hasbegantobeusedintheexperimentofischemiccerebrovasculardisease，butitisstillnotclearabouttherelationshipbetweenIGF-1andtheexpressionofVEGFandbFGFandtheexactneurotrophicprotectivemechanismofIGF一1afterce
6、rebralischemiaSOfar．Theratmodelofmiddlecerebralarteryocclusion(MCAO)andreperfusionwasestablishedwithsutureembolimethod．5英文摘要AfterinjectingIGF-1vialateralcerebralventricle，theIGF一1’SeffectontheexpressionofVEGFandbFGFbothongeneandproteinlevelwasobservedthroughRT-PCRandimmunohisto—chemistrymethods．In
7、thisstudy,wepreliminarilydiscussedthepossiblemolecularmechanismofIGF-1’sprotectiveeffectstowardscerebralischemicdamageandprovidedanewtheoreticalbasisforIGF一1’Sfurtherclinicalapplicationandanewwayfortheclinicaltre
