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1、中國(guó)修復(fù)重建外科雜志2008年2月第22卷第2期·145·組織工程骨膜異體體內(nèi)成骨修復(fù)兔骨缺損的初步觀察123311趙琳史志勇周晟賈有福劉佳王軍勝【摘?要】目的探討以兔BMSCs和豬SIS復(fù)合構(gòu)建的組織工程骨膜，在異體兔體內(nèi)成骨的可行性。方法取新西蘭大白兔BMSCs與SIS復(fù)合構(gòu)建組織工程骨膜。選2月齡新西蘭大白兔12只，制備雙側(cè)橈骨1.5～2.0cm缺損模型。隨機(jī)選一側(cè)植入組織工程骨膜,作為實(shí)驗(yàn)組；另一側(cè)僅植入單純SIS，作為對(duì)照組。術(shù)后觀察動(dòng)物一般情況，4周后攝X線片觀察，取骨缺損中段標(biāo)本行HE及Masson染色觀察。結(jié)果
2、兩組動(dòng)物術(shù)后飲食及日常活動(dòng)基本正常；傷口無紅腫、溢膿等；傷肢基本能負(fù)重行走。X線片觀察：實(shí)驗(yàn)組骨缺損處有長(zhǎng)條狀新生骨形成，密度與正常骨相同，新生骨橋接骨缺損；對(duì)照組骨缺損無骨形成征象，骨缺損處密度與周圍軟組織影相近。組織學(xué)觀察：實(shí)驗(yàn)組骨缺損處有新骨形成，骨組織中可見血管腔及不規(guī)則髓腔樣結(jié)構(gòu)；未見明顯異物巨噬細(xì)胞及淋巴細(xì)胞浸潤(rùn)征象；對(duì)照組骨缺損處僅為膠原瘢痕組織，無骨組織形成。結(jié)論以SIS和BMSCs構(gòu)建的組織工程骨膜在同種異體體內(nèi)可以成骨，有修復(fù)骨缺損的可行性?！娟P(guān)鍵詞】??組織工程骨膜??BMSCs??SIS??骨缺損兔中圖
3、分類號(hào)：R318.08?Q813?文獻(xiàn)標(biāo)志碼：ATHEPRIMARYOBSERVATIONOFTISSUEENGINEEREDPERIOSTEUMOSTEOGENESISINVIVOINALLO-GENICRABBIT/ZHAOLin1,SHIZhiyong2,ZHouShen3,JIAYoufu3,LIUJia1,WANGJunsheng1.1DepartmentofOrthope-dics,the2ndhospitalofLanzhouuniversity,LanzhouGansu,730030,P.R.China;2Th
4、eExperimentalAnimalCenterofGeneralHospitalofLanzhouMilitaryRegion;3DepartmentofRadiology,ChinesetraditionalMedicinehospitalofGansuProvince.Cor-respondingauthor：ZHAOLin,E-mail：bonezl@sina.com【Abstract】Objective?Toinvestigatetheinvivoosteogenicfeasibilityoftissueengin
5、eeredperiosteumconstructedbyporcineSISandBMSCsinallogenicNewZealandrabbit.?MethodsThetissueengineeredperiosteumconstructedbySISscaffoldandBMSCswaspreparedinvitro.Twelve2-month-oldNewZealandrabbitswereusedintheexperiments.The1.5-2.0cmcriticalbonedefectsweremadeintheb
6、othsidesofradiusoftheanimals.Thetissueengineeredperiosteumwasgraftedintoonesidedefectrandomly,whiletheothersidedefectwasonlygraftedSIS.Fourweeksafteroperation,theforearmsofallanimalswerecheckedbyX-ray.Then,animalsweresacrificedtoharvestthespecimenwhichweretreatedpro
7、mptlyforHEandMassonstaining.TheX-rayfilmandthemorphologicaltissuestainingoutcomewereevaluatedqualitatively.?Results?Afteroperation,allanimalshadanormalbehavioranddiet;theincisionhealednormally;theforearmcouldmovenormallyforbearingweight.Thetissueengineeredperiosteum
8、constructedbyallogenicBMSCsandheterogeneicSISscaffoldcouldformnewbonetissue,andbridgedthebonedefectwhichcouldbeconfirmedeitherinX-rayfilmo
