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1、中國修復重建外科雜志2008年2月第22卷第2期·145·組織工程骨膜異體體內(nèi)成骨修復兔骨缺損的初步觀察123311趙琳史志勇周晟賈有福劉佳王軍勝【摘?要】目的探討以兔BMSCs和豬SIS復合構(gòu)建的組織工程骨膜，在異體兔體內(nèi)成骨的可行性。方法取新西蘭大白兔BMSCs與SIS復合構(gòu)建組織工程骨膜。選2月齡新西蘭大白兔12只，制備雙側(cè)橈骨1.5～2.0cm缺損模型。隨機選一側(cè)植入組織工程骨膜,作為實驗組；另一側(cè)僅植入單純SIS，作為對照組。術后觀察動物一般情況，4周后攝X線片觀察，取骨缺損中段標本行HE及Masson染色觀察。結(jié)果
2、兩組動物術后飲食及日?；顒踊菊＃粋跓o紅腫、溢膿等；傷肢基本能負重行走。X線片觀察：實驗組骨缺損處有長條狀新生骨形成，密度與正常骨相同，新生骨橋接骨缺損；對照組骨缺損無骨形成征象，骨缺損處密度與周圍軟組織影相近。組織學觀察：實驗組骨缺損處有新骨形成，骨組織中可見血管腔及不規(guī)則髓腔樣結(jié)構(gòu)；未見明顯異物巨噬細胞及淋巴細胞浸潤征象；對照組骨缺損處僅為膠原瘢痕組織，無骨組織形成。結(jié)論以SIS和BMSCs構(gòu)建的組織工程骨膜在同種異體體內(nèi)可以成骨，有修復骨缺損的可行性?！娟P鍵詞】??組織工程骨膜??BMSCs??SIS??骨缺損兔中圖
3、分類號：R318.08?Q813?文獻標志碼：ATHEPRIMARYOBSERVATIONOFTISSUEENGINEEREDPERIOSTEUMOSTEOGENESISINVIVOINALLO-GENICRABBIT/ZHAOLin1,SHIZhiyong2,ZHouShen3,JIAYoufu3,LIUJia1,WANGJunsheng1.1DepartmentofOrthope-dics,the2ndhospitalofLanzhouuniversity,LanzhouGansu,730030,P.R.China;2Th
4、eExperimentalAnimalCenterofGeneralHospitalofLanzhouMilitaryRegion;3DepartmentofRadiology,ChinesetraditionalMedicinehospitalofGansuProvince.Cor-respondingauthor：ZHAOLin,E-mail：bonezl@sina.com【Abstract】Objective?Toinvestigatetheinvivoosteogenicfeasibilityoftissueengin
5、eeredperiosteumconstructedbyporcineSISandBMSCsinallogenicNewZealandrabbit.?MethodsThetissueengineeredperiosteumconstructedbySISscaffoldandBMSCswaspreparedinvitro.Twelve2-month-oldNewZealandrabbitswereusedintheexperiments.The1.5-2.0cmcriticalbonedefectsweremadeintheb
6、othsidesofradiusoftheanimals.Thetissueengineeredperiosteumwasgraftedintoonesidedefectrandomly,whiletheothersidedefectwasonlygraftedSIS.Fourweeksafteroperation,theforearmsofallanimalswerecheckedbyX-ray.Then,animalsweresacrificedtoharvestthespecimenwhichweretreatedpro
7、mptlyforHEandMassonstaining.TheX-rayfilmandthemorphologicaltissuestainingoutcomewereevaluatedqualitatively.?Results?Afteroperation,allanimalshadanormalbehavioranddiet;theincisionhealednormally;theforearmcouldmovenormallyforbearingweight.Thetissueengineeredperiosteum
8、constructedbyallogenicBMSCsandheterogeneicSISscaffoldcouldformnewbonetissue,andbridgedthebonedefectwhichcouldbeconfirmedeitherinX-rayfilmo
