大豆異黃酮誘導HeLa細胞凋亡、細胞周期阻滯和[Ca^2＋]i濃度升高-論文.pdf

《大豆異黃酮誘導HeLa細胞凋亡、細胞周期阻滯和[Ca^2＋]i濃度升高-論文.pdf》由會員上傳分享，免費在線閱讀，更多相關內(nèi)容在行業(yè)資料-天天文庫。

1、營養(yǎng)學報2012年第34卷第4期373SOYISOFLAVONESINDUCEDAPOPTOSIS，CELLCYCLEARRESTAND[CA]ELEVAT10NINHELACELLSXIAOJun—xia，HUANGGuo—qing，CHIYu·sen，WANGShi—qing(CollegeofFoodScienceandEngineering,QingdaoAgriculturalUniversity,Qingdao266109，Chin[Abstract]ObjectiveToinvestigatethegrow

2、thinhibitionmechanismofsoyisoflavonesmixtureSI—IonHelacells．MethodAfterexposureto10，20，and40gg／mlSI—Ifor4d，HeLacellswereexaminedwithinvertedmicroscopeandtransmissionelectronmicroscope．Thecellcycledistributionwasanalyzedbyflowcytometryandintracellularcalciumions([

3、Ca]i)detectedbyconfocallaserscanningmicroscope．ResultsTheinvertedmicroscopyshowedslowercellsgrowth，andobviousmorphologicalchanges．ThetransmissionelectronmicroscopyrevealedthatSI—IinhibitedHelacellgrowthbyinducingapoptosis．Theflowcytometryshowedthatthecellcyclewas

4、arrestedinG0／G1orGjMphasedependingonSI-Iconcentrationandtheproportionsofapoptosiscellsincreasedlinearly．Confocallaserscanningmicroscopeanalysisdemonstratedasignificantincreasein[Ca]iinSI—ItreatedHeLacells．ConclusionSI·IinhibitedHeLacellgrowththroughapoptosisinduc

5、tionandcellcyclearrestandtheincreaseofintracellular[Ca]Jmightbethemechanismsofapoptosis．]ACTANUTRIMENTASINICA，2012，34(4)：373—3781Keywords：soyisoflavones；Helacells；apoptosis；cellcycle；intracellularcalciumions大百丌里黃酮誘導HeLa細胞凋亡、細胞周期阻滯和[Ca2+]i濃度升．同_C_jI—肖軍霞，黃國清，遲玉森，王世

6、清(青島農(nóng)業(yè)大學食品科學與工程學院，青島266109)【摘要l目的探討大豆異黃酮sI—I抑制HeLa細胞生長機制。方法HeLa細胞經(jīng)過10，20，and40btg／ml的大豆異黃酮sI—I處理4d后，采用倒置顯微鏡和透射電鏡觀察其形態(tài)學變化，利用流式細胞儀檢測細胞周期分布，通過激光掃描共聚焦顯微鏡觀察細胞中[ca】濃度。結(jié)果倒置顯微鏡觀察到HeLa細胞數(shù)量明顯變少，細胞嚴重變形。透射電鏡觀察到HeLa細胞發(fā)生凋亡形態(tài)學變化。流式細胞儀分析發(fā)現(xiàn)HeLa細胞周期阻滯于G／G或G／M期，細胞凋亡率隨sI—I濃度的增加而上升。激

7、光掃描共聚焦顯微鏡觀察到凋亡的HeLa細胞內(nèi)[Ca】．顯著升高。結(jié)論大豆異黃酮sI—I抑制HeLa細胞生長是通過凋亡誘導作用，并且細胞周期阻滯和【ca]，濃度升高可能是其誘導凋亡機制之一。[營養(yǎng)學報，2012，34(4)：373—378]關鍵詞：大豆異黃酮；HeLa細胞；凋亡；細胞周期；細胞內(nèi)鈣離子中圖分類號：R151．1文獻標識碼：A文章編號：0512—7955(2012)040373—061lNTRoDUCTIoNEcompounds，ofwhich，genistein(5,7，4一trihydroxyi—Epide

8、miologicstudiesindicatethatthesoflavone)anddaidzein(7,4一dihydroxyisonavone)，consum·-ptionofsoy-containingdietsisassociatedarelikelypredominantinthecancerpreven