大豆異黃酮誘導(dǎo)HeLa細(xì)胞凋亡、細(xì)胞周期阻滯和[Ca^2＋]i濃度升高-論文.pdf

《大豆異黃酮誘導(dǎo)HeLa細(xì)胞凋亡、細(xì)胞周期阻滯和[Ca^2＋]i濃度升高-論文.pdf》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。

1、營養(yǎng)學(xué)報2012年第34卷第4期373SOYISOFLAVONESINDUCEDAPOPTOSIS，CELLCYCLEARRESTAND[CA]ELEVAT10NINHELACELLSXIAOJun—xia，HUANGGuo—qing，CHIYu·sen，WANGShi—qing(CollegeofFoodScienceandEngineering,QingdaoAgriculturalUniversity,Qingdao266109，Chin[Abstract]ObjectiveToinvestigatethegrow

2、thinhibitionmechanismofsoyisoflavonesmixtureSI—IonHelacells．MethodAfterexposureto10，20，and40gg／mlSI—Ifor4d，HeLacellswereexaminedwithinvertedmicroscopeandtransmissionelectronmicroscope．Thecellcycledistributionwasanalyzedbyflowcytometryandintracellularcalciumions([

3、Ca]i)detectedbyconfocallaserscanningmicroscope．ResultsTheinvertedmicroscopyshowedslowercellsgrowth，andobviousmorphologicalchanges．ThetransmissionelectronmicroscopyrevealedthatSI—IinhibitedHelacellgrowthbyinducingapoptosis．Theflowcytometryshowedthatthecellcyclewas

4、arrestedinG0／G1orGjMphasedependingonSI-Iconcentrationandtheproportionsofapoptosiscellsincreasedlinearly．Confocallaserscanningmicroscopeanalysisdemonstratedasignificantincreasein[Ca]iinSI—ItreatedHeLacells．ConclusionSI·IinhibitedHeLacellgrowththroughapoptosisinduc

5、tionandcellcyclearrestandtheincreaseofintracellular[Ca]Jmightbethemechanismsofapoptosis．]ACTANUTRIMENTASINICA，2012，34(4)：373—3781Keywords：soyisoflavones；Helacells；apoptosis；cellcycle；intracellularcalciumions大百丌里黃酮誘導(dǎo)HeLa細(xì)胞凋亡、細(xì)胞周期阻滯和[Ca2+]i濃度升．同_C_jI—肖軍霞，黃國清，遲玉森，王世

6、清(青島農(nóng)業(yè)大學(xué)食品科學(xué)與工程學(xué)院，青島266109)【摘要l目的探討大豆異黃酮sI—I抑制HeLa細(xì)胞生長機制。方法HeLa細(xì)胞經(jīng)過10，20，and40btg／ml的大豆異黃酮sI—I處理4d后，采用倒置顯微鏡和透射電鏡觀察其形態(tài)學(xué)變化，利用流式細(xì)胞儀檢測細(xì)胞周期分布，通過激光掃描共聚焦顯微鏡觀察細(xì)胞中[ca】濃度。結(jié)果倒置顯微鏡觀察到HeLa細(xì)胞數(shù)量明顯變少，細(xì)胞嚴(yán)重變形。透射電鏡觀察到HeLa細(xì)胞發(fā)生凋亡形態(tài)學(xué)變化。流式細(xì)胞儀分析發(fā)現(xiàn)HeLa細(xì)胞周期阻滯于G／G或G／M期，細(xì)胞凋亡率隨sI—I濃度的增加而上升。激

7、光掃描共聚焦顯微鏡觀察到凋亡的HeLa細(xì)胞內(nèi)[Ca】．顯著升高。結(jié)論大豆異黃酮sI—I抑制HeLa細(xì)胞生長是通過凋亡誘導(dǎo)作用，并且細(xì)胞周期阻滯和【ca]，濃度升高可能是其誘導(dǎo)凋亡機制之一。[營養(yǎng)學(xué)報，2012，34(4)：373—378]關(guān)鍵詞：大豆異黃酮；HeLa細(xì)胞；凋亡；細(xì)胞周期；細(xì)胞內(nèi)鈣離子中圖分類號：R151．1文獻(xiàn)標(biāo)識碼：A文章編號：0512—7955(2012)040373—061lNTRoDUCTIoNEcompounds，ofwhich，genistein(5,7，4一trihydroxyi—Epide

8、miologicstudiesindicatethatthesoflavone)anddaidzein(7,4一dihydroxyisonavone)，consum·-ptionofsoy-containingdietsisassociatedarelikelypredominantinthecancerpreven