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1、256董明，等黃芩苷對肝癌細(xì)胞株SMMC一7721裸鼠移植瘤生長抑制作用及其機(jī)銅黃芩苷對肝癌細(xì)胞株SMMC一7721裸鼠移植瘤生長抑制作用及其機(jī)制董明，侯俊明，高美花，魏輝ExperimentalstudyontheefectofBaicalinonCyclinD1andCaspase一3activityofhumanhepatocellularcarcinomaSMMC一7721celinnudemiceDongMing，HouJunming，GaoMeihua~，WeiHui、AfiliatedHospimlofShaan
2、xiUniversity。fChineseMedicine，ShaanxiXⅫ1712000，ChinaAffiliatedHospitalofYanbianUniversity，問inYanbian133000，China【Abstract】Objective：TotesttheefectofBaicalinongrowthoftransplantationtmnorusingtheSMMC一7721ani—malmodelinnudemicebearinghmnanhepatocellularcarcinonlacelll
3、ineMethods：BALB／cnudemicewereinjee—tedsubcutaneouslywithhmnanhepatolnacellineSMMC一77215×10／mlforduplicatetmnormodel20transplan—tednudemicewererandomlydividedintoblankcontrolgroupatdbaicalingroup，10ratsineachgroupThecontrolgroupwasgivennorlnalsalinebyintraperitoneali
4、njectionof10nfl／kg，Baicalinintraperitonealinjectionof100mkg，every2dinterlnitentdosingweightafter8tilnes，puttmnorweig，cancerinhibitionratetmnorCyclinD1andtheex—pressionofCaspase一3levelswerel~leasHredbyilnlnunohistochelnistryResults：Baicalin100mkgofthedorsmnSMMC一7721s
5、olidtmnoroflivercancerinhibitionratewas308％．theindexofliverandkidney．betweenthetwogroupswassimilar(P>005)，thesuppressionofCyclinD1expressionandraisedintheexpressionofCaspase一3(P<005)Condusion：BaicalininhumanliverSMMC一7721ofthedorsmnsubcutaneoustransplantationtmnorgr
6、owthinhibition，itsefectsmaycutCyclinD1expressionthroughorincreasetheexpressionofCaspase一3occlusiontoin—hibitproliferationandpromotingthefunctionofapoptosisintheprotectionofLllcorgansfunction．baicalinhasnoobviousadvantageBaicalinmaybecomeoneoftheefectivetreatlnentfnp
7、rimaryhepatocelularcarcinonlafHCC)【Keywords】Baicalin；humanhepatolnacellineSMMC一7721；CyclinD1；Caspase一3ModernOncology2014，22(02)：0256—0258【摘要】目的：以裸鼠人肝癌細(xì)胞株SMMC一7721移植瘤動物模型為研究對象，觀察黃芩苷對移植瘤生長能力的影響，初步探討黃芩苷對CyclinD1和Caspase一3表達(dá)的影響。方法：BALB／c裸鼠右前腋部皮下接種人肝癌SMMC一7721細(xì)胞5×10個，建立人
8、肝癌實體瘤模型。將20只成瘤裸小鼠隨機(jī)分為空白對照組與黃芩苷組，每組l0只。對照組腹腔注射生理鹽水10ml／kg，黃芩苷組腹腔注射黃芩苷注射液lOOmkg，每2d給藥1次，間斷給藥8次后稱體重、處死，瘤塊離體稱重，計算抑瘤率；用免疫組織化學(xué)方法檢測實驗組和對照組移植瘤中Cyc
