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《sirna蛋白抑制對(duì)lncap細(xì)胞增殖的影響作用》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在教育資源-天天文庫(kù)。
1、siRNA蛋白抑制對(duì)LNCaP細(xì)胞增殖的影響作用siRNA???????.??LNCaP?????????????????g????H???????H??/???kH?????H?????H???????H?????HT????HeH?????H????P????R??q??2A??????(AMACR)???O?L??????RNA(siRNA),?????????????.?3?????W??????????LNCaP??????????HiH?????H????????a?T3??????AMACR??siRNA???W,?n??siPORTTMNeoFXTM????LNC
2、aP????;RT2PCR????siRNA???g?3AMACRmRNA??????,Westernblot????AMACR????????,CCK28????????????????,?w?O?????G????????????HiH?????H??n?????????h?????j???L??,?m??21030??siRNA???P?/???.AMACR??????77%,?P?/???.AM2ACRmRNA?y????60%,?m???k113442??21203??siRNA?V?,???P?/???.AMACR????Hi?????m??21030??siRNA?
3、????.LNCaP????????,?]?d?????3?9??????????HiH?????H??m??21030??siRNA?????i?P?/?M?RLNCaPAMACR????????,?????????.?3?????.LNCaP????????,?k???W?????????????????????K??HiHT???Y??HeH????W?????6;H???;H??O?L??????RNA;H?????????ThescreeningofsiRNAtoAMACRandtheinfluenceofLNCaPH?JIYong3,WANGXiao2feng,CHE
4、NGuo2qiang,JINBing,YANGBo,HEPei2ying,YANZheng.3DepartmentofUrology,People1rsHospital,PekingUniversity,Beijing100044,ChinaCorrespondingauthor:JIYong,Email:beaver1999@sohu.comHTAbstractHeH?ObjectiveH?ToscreenasiRNAtoAMACR,andtoinvestigatetheproliferationinfluenceofprostatecancercelllineLNCaPaft
5、ertheproteininhibition.H?MethodsH?Designandsynthesizationof3AMACRsiRNAsequences,andLNCaPcellsweretransfectedbysiPORTTMNeoFXTM;theexpressionofAM2ACRmRNAaftersiRNAactionwasdetectedbyRT2PCR;theexpressionofAMACRproteinwasdetectedbyWesternblot;theproliferationwasexaminedwithCCK28;flowcytometrydete
6、ctedcellapoptosis.H?ResultsH?Thenumber21030siRNAcouldeffectivelyinhibitAMACRproteinexpressionabout77%andAMACRmR2NAlevelabout60%,whilenumber113442and21203siRNAscouldnoteffectivelyinhibitAMACRexpres2sion.TheproliferationofLNCaPcellswasblockedafterthenumber21030siRNAtransfection,butnoapopto2sish
7、appened.H?ConclusionsH?Number21030siRNAcouldeffectivelyandspecificallydown2regulateLNCaPAMACRgeneexpression.TheproteininhibitioncanblocktheproliferationofLNCaPcellsthatmayprovideanewwayfortheprostatecancergenetherapy.HTKeywordsHeH?Prostaticne
