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1、涼血解毒方治療銀屑病的實(shí)驗(yàn)研究作者:顧敏婕,高尚璞,李詠梅【摘要】目的:觀察涼血解毒方對(duì)小鼠尾部有顆粒層的鱗片和陰道上皮細(xì)胞有絲分裂的影響,探討其治療銀屑病的可能作用機(jī)制。方法:選取60只小鼠隨機(jī)分為涼血解毒方組、復(fù)方青黛膠囊組、阿維A膠囊組和生理鹽水組,每組15只,另選10只正常小鼠作為空白對(duì)照組。按人鼠等效劑量連續(xù)灌胃相應(yīng)藥物14d,1次/d,第15天處死,取材,蘇木精和伊紅染色法檢測(cè)并計(jì)算尾部表皮細(xì)胞中顆粒細(xì)胞百分比。隨機(jī)選取60只雌性小鼠,腹腔注射乙烯雌酚3d后分為上述4組,繼續(xù)腹腔注射乙烯雌酚。另選用10只正常
2、小鼠作為對(duì)照組。分組和灌胃方法同前。第15天小鼠腹腔注射秋水仙堿,6h后處死,取材,HE染色法觀察并計(jì)算小鼠陰道上皮細(xì)胞有絲分裂指數(shù)。結(jié)果:與生理鹽水組比較,涼血解毒方組小鼠尾部鱗片顆粒細(xì)胞增加(P【關(guān)鍵詞】涼血解毒方;銀屑病;有絲分裂;小鼠Objective:TostudytheeffectsofLiangxueJieduDecoction,acompoundtraditionalChineseherbalmedicinewiththefunctionofbloodcoolinganddetoxicating,intr
3、eatingpsoriasisinmiceandtoexploreitsmechanism.Methods:(1)Sixtymicewererandomlydividedint涼血解毒方治療銀屑病的實(shí)驗(yàn)研究作者:顧敏婕,高尚璞,李詠梅【摘要】目的:觀察涼血解毒方對(duì)小鼠尾部有顆粒層的鱗片和陰道上皮細(xì)胞有絲分裂的影響,探討其治療銀屑病的可能作用機(jī)制。方法:選取60只小鼠隨機(jī)分為涼血解毒方組、復(fù)方青黛膠囊組、阿維A膠囊組和生理鹽水組,每組15只,另選10只正常小鼠作為空白對(duì)照組。按人鼠等效劑量連續(xù)灌胃相應(yīng)藥物14d,1次/d,
4、第15天處死,取材,蘇木精和伊紅染色法檢測(cè)并計(jì)算尾部表皮細(xì)胞中顆粒細(xì)胞百分比。隨機(jī)選取60只雌性小鼠,腹腔注射乙烯雌酚3d后分為上述4組,繼續(xù)腹腔注射乙烯雌酚。另選用10只正常小鼠作為對(duì)照組。分組和灌胃方法同前。第15天小鼠腹腔注射秋水仙堿,6h后處死,取材,HE染色法觀察并計(jì)算小鼠陰道上皮細(xì)胞有絲分裂指數(shù)。結(jié)果:與生理鹽水組比較,涼血解毒方組小鼠尾部鱗片顆粒細(xì)胞增加(P【關(guān)鍵詞】涼血解毒方;銀屑病;有絲分裂;小鼠Objective:TostudytheeffectsofLiangxueJieduDecoction,ac
5、ompoundtraditionalChineseherbalmedicinewiththefunctionofbloodcoolinganddetoxicating,intreatingpsoriasisinmiceandtoexploreitsmechanism.Methods:(1)SixtymicewererandomlydividedintoLiangxueJieduDecodlndigoNaturaliscaincapsulegroupandnnotherlOmiceweresectiongroup,comp
6、ounpsulegroup,acitretormalsalinegroup.Alectedasblankcontrol.After2nsamples.Afterhematoxy1inandeosin(HEeswithgranulariayeanopticalmicroscopiceinblankgroup,sinjectedintraperito)staining,tailscalrswerecalculatedbye.(2)Exceptfortenmxtyfemalemicewereineallywithdiethyl
7、stilbestroloncedaily.After3dayinjection,micewererandomlydividedintofourgweekadministration,miceweresacrificedtoobtairoupsandtreatedasabovedescription.After2weektreatment,allmicewereinjectedintraperitoneallywithcolchicine(2mg/kg),andsacrificed6haftertheinjection.T
8、hemitoticrateinvirginalepitheliumwascalculatedafterHEstaining.Results:Comparedwithnormalsaline,LiangxueJieduDecoctioncouldsignificantlyinhibitthemitosisofmoum(