抗?jié)h坦病毒單鏈抗體2fcκ2fprotamine截短體融合蛋白的基因構(gòu)建、表達(dá)與功能的研究

抗?jié)h坦病毒單鏈抗體2fcκ2fprotamine截短體融合蛋白的基因構(gòu)建、表達(dá)與功能的研究

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抗?jié)h坦病毒單鏈抗體2fcκ2fprotamine截短體融合蛋白的基因構(gòu)建、表達(dá)與功能的研究_第1頁
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1、獨(dú)創(chuàng)性聲明秉承學(xué)校嚴(yán)謹(jǐn)?shù)膶W(xué)風(fēng)與優(yōu)良的科學(xué)道德,本人聲明所呈交的論文是我個(gè)人在導(dǎo)師指導(dǎo)下進(jìn)行的研究工作及取得的研究成果。盡我所知,除了文中特別加以標(biāo)注和致謝的地方外,論文中不包含其他人已經(jīng)發(fā)表或撰寫過的研究成果,不包含本人或他人已申請學(xué)位或其他用途使用過的成果。與我一同工作的同志對本研究所做的任何貢獻(xiàn)均已在論文中作了明確的說明并表示了致謝。申請學(xué)位論文與資料若有不實(shí)之處,本人承擔(dān)一切相關(guān)責(zé)任。論文作者簽名:日期:保護(hù)知識產(chǎn)權(quán)聲明本人完全了解第四軍醫(yī)大學(xué)有關(guān)保護(hù)知識產(chǎn)權(quán)的規(guī)定,即:研究生在校攻讀學(xué)位期間論文工作的知識產(chǎn)權(quán)單位屬第四軍醫(yī)大學(xué)。本人保證畢

2、業(yè)離校后,發(fā)表論文或使用論文工作成果時(shí)署名單位仍然為第四軍醫(yī)大學(xué)。學(xué)??梢怨颊撐牡娜炕虿糠謨?nèi)容(含電子版,保密內(nèi)容除外),可以采用影印,縮印或其他復(fù)制手段保存論文。學(xué)校有權(quán)允許論文被查閱和借閱,并在校園網(wǎng)上提供論文內(nèi)容的瀏覽和下載服務(wù)。論文作者簽名:導(dǎo)師簽名:日期:抗?jié)h坦病毒單鏈抗體/Cκ/protamine截短體融合蛋白的基因構(gòu)建、表達(dá)及功能研究研究生:陳銳學(xué)科專業(yè):生物化學(xué)與分子生物學(xué)所在單位:第四軍醫(yī)大學(xué)生物化學(xué)與分子生物學(xué)教研室導(dǎo)師:楊安鋼(教授)輔導(dǎo)教師:楊潔(講師)資助基金項(xiàng)目:國家973計(jì)劃項(xiàng)目2004CB518805軍隊(duì)“十一

3、五”科技攻關(guān)項(xiàng)目(06G084)教育部“長江學(xué)者和創(chuàng)新團(tuán)隊(duì)發(fā)展計(jì)劃”創(chuàng)新團(tuán)隊(duì)IRT0459關(guān)鍵詞:HV;單鏈抗體;魚精蛋白;靶向輸送中國人民解放軍第四軍醫(yī)大學(xué)2008年05月第四軍醫(yī)大學(xué)碩士學(xué)位論文目錄縮略語表·····················································································································1中文摘要·················································

4、·········································································3Abstract·····························································································································5前言··································································

5、························································7文獻(xiàn)回顧·····················································································································9(一)、漢坦病毒(HV)感染疾病治療研究進(jìn)展·····················································9(二)、單鏈抗體融合蛋白在生物治療中的應(yīng)用

6、····················································12(三)、靶向性非病毒載體系統(tǒng)介導(dǎo)的基因轉(zhuǎn)移····················································17(四)、siRNA的研究進(jìn)展························································································21正文·········································

7、···············································································24第一部分抗HV單鏈抗體/Cκ/protamine截短體融合蛋白的基因構(gòu)建·································241.實(shí)驗(yàn)材料······································································································241.1菌種與質(zhì)?!ぁぁぁぁぁ?/p>

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