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1、Abstractwim26bacteriastrainsshowedthatonlyStaphylococcusaureus(CMCC26003)andSalmonellatyphimurium(ATCC13311)hadslightcrossreaction，whereastherest24strainshadnocrossreaction．Thepreservationtestat37"Cindicatedthatthestripscouldbestoredatroomtemperatureformorethan15montllS．Th
2、estripsshowedpositiveresultafterinoculatingEcoliO157：H7insamples(beeforlettuce)at10to100CFU／25gandincubatingfor6h．Inordertoincreasethespecificityandsensitivityoftheassay，Balb／cmicewereimmunizedwithflagellumproteinsisolatedfromEcoliO157：H7．Afterfusionandcloning，fourhybridom
3、acellswhichwouldstablesecretantibodyS_againstEcoli0157：H7wereabtainedandpreparedasciticfluid．Thematchingresultsshowedthat5pairsofantibodiescouldbeusedforstrippreparation．Keywords：EscherichiacoliO157：H7；polyclonalantibody；colloidalgold；immunochromatography；monoclonalantibod
4、yIII縮略語(yǔ)表IV縮略語(yǔ)表目錄摘j19l：???????．???．??．?．?．???．??．?．???．?．???．?．．???．??．?．??IABSTRACT?????????????????????????????????????．II縮略語(yǔ)表??????????????????????????????IV第一章引言????????????????????????????．．11．1Ecoli0157：H7的臨床表現(xiàn)和感染?????????????????11．2Ecoli0157：H7的特異性抗原和抗體制備研究進(jìn)展???????
5、???21．2．1Ecoli0157：H7的O抗原?????????????????????????．21．2．2Ecoli0157：H7的H抗原?????????????????????????．21．2．3其它特異性抗原???????????????????????．31．3Ecoli0157：H7檢測(cè)方法的研究進(jìn)展????????????????41．3．1常規(guī)檢測(cè)方法????????????????????????．41．3．2基于PCR的分子生物學(xué)方法?????????????????．．51．3．3免疫學(xué)檢測(cè)方法????????
6、???????????????．61．3．4新興技術(shù)??????????????????????????．．71．4膠體金快速檢測(cè)技術(shù)的概述???????????????????．71．4．1膠體金的性質(zhì)????????????????????????81．4．2膠體金制備方法???????????????????????81．4-3膠體金標(biāo)記抗體???????????????????????81．4．4免疫膠體技術(shù)在微生物檢測(cè)中的應(yīng)用????????????k??91．5展望????????????????????????????????
7、??????．9第二章抗大腸桿菌0157：H7多克隆抗體的制備?????????????lO2．1前言??????????????????????????????????????．102．2材料與方法??????????????????????????102．2．1菌株、免疫動(dòng)物???????????????????????102．2．2主要試劑和儀器設(shè)備????????????????????．．102．2．3相關(guān)試劑和培養(yǎng)基配置????????????????????102．2．4Ecob0157：H7抗原制備?????????????
8、??????112．2．5其它菌株包被原的制備????????????????????122．2．6動(dòng)物免疫??????????????????????????122．2．7間接
