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1、Abstractwim26bacteriastrainsshowedthatonlyStaphylococcusaureus(CMCC26003)andSalmonellatyphimurium(ATCC13311)hadslightcrossreaction,whereastherest24strainshadnocrossreaction.Thepreservationtestat37"Cindicatedthatthestripscouldbestoredatroomtemperatureformorethan15montllS.Th
2、estripsshowedpositiveresultafterinoculatingEcoliO157:H7insamples(beeforlettuce)at10to100CFU/25gandincubatingfor6h.Inordertoincreasethespecificityandsensitivityoftheassay,Balb/cmicewereimmunizedwithflagellumproteinsisolatedfromEcoliO157:H7.Afterfusionandcloning,fourhybridom
3、acellswhichwouldstablesecretantibodyS_againstEcoli0157:H7wereabtainedandpreparedasciticfluid.Thematchingresultsshowedthat5pairsofantibodiescouldbeusedforstrippreparation.Keywords:EscherichiacoliO157:H7;polyclonalantibody;colloidalgold;immunochromatography;monoclonalantibod
4、yIII縮略語(yǔ)表IV縮略語(yǔ)表目錄摘j19l:???????.???.??.?.?.???.??.?.???.?.???.?..???.??.?.??IABSTRACT?????????????????????????????????????.II縮略語(yǔ)表??????????????????????????????IV第一章引言????????????????????????????..11.1Ecoli0157:H7的臨床表現(xiàn)和感染?????????????????11.2Ecoli0157:H7的特異性抗原和抗體制備研究進(jìn)展???????
5、???21.2.1Ecoli0157:H7的O抗原?????????????????????????.21.2.2Ecoli0157:H7的H抗原?????????????????????????.21.2.3其它特異性抗原???????????????????????.31.3Ecoli0157:H7檢測(cè)方法的研究進(jìn)展????????????????41.3.1常規(guī)檢測(cè)方法????????????????????????.41.3.2基于PCR的分子生物學(xué)方法?????????????????..51.3.3免疫學(xué)檢測(cè)方法????????
6、???????????????.61.3.4新興技術(shù)??????????????????????????..71.4膠體金快速檢測(cè)技術(shù)的概述???????????????????.71.4.1膠體金的性質(zhì)????????????????????????81.4.2膠體金制備方法???????????????????????81.4-3膠體金標(biāo)記抗體???????????????????????81.4.4免疫膠體技術(shù)在微生物檢測(cè)中的應(yīng)用????????????k??91.5展望????????????????????????????????
7、??????.9第二章抗大腸桿菌0157:H7多克隆抗體的制備?????????????lO2.1前言??????????????????????????????????????.102.2材料與方法??????????????????????????102.2.1菌株、免疫動(dòng)物???????????????????????102.2.2主要試劑和儀器設(shè)備????????????????????..102.2.3相關(guān)試劑和培養(yǎng)基配置????????????????????102.2.4Ecob0157:H7抗原制備?????????????
8、??????112.2.5其它菌株包被原的制備????????????????????122.2.6動(dòng)物免疫??????????????????????????122.2.7間接