產(chǎn)黃纖維單胞菌CR-14菌株誘變選育及發(fā)酵條件優(yōu)化

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1、農(nóng)業(yè)資源與環(huán)境學(xué)報JournalofAgriculturalResourcesandEnvironment2016年5月·第33卷·第3期：262-267May2016·Vol.33·No.3：262-267產(chǎn)黃纖維單胞菌CR-14菌株誘變選育及發(fā)酵條件優(yōu)化*楊繼業(yè)，崔冠慧，習(xí)彥花，李亞冰，張根偉，張麗萍，程輝彩（河北省科學(xué)院生物研究所，河北石家莊050081）摘要：為提高產(chǎn)黃纖維單胞菌CR-14纖維素酶活力，對菌株進(jìn)行亞硝酸、紫外線及復(fù)合誘變處理，篩選產(chǎn)纖維素酶活較高的突變株，并對其發(fā)酵條件進(jìn)行優(yōu)化。結(jié)

2、果表明，經(jīng)亞硝酸和紫外線復(fù)合誘變得到一株產(chǎn)纖維素酶活較高的菌株Y-UA-18，其酶活力為10.57U，為原菌株產(chǎn)酶活力的1.67倍。通過正交試驗得到菌株Y-UA-18產(chǎn)酶最佳培養(yǎng)基為：秸稈粉1.0%、復(fù)合氮源0.6%、KH2PO40.1%、MgSO40.1%、NaCl0.08%；最佳培養(yǎng)條件為：初始pH值7.0、培養(yǎng)溫度30℃、發(fā)酵時間3d。通氣量對菌株Y-UA-18產(chǎn)酶影響不明顯，但在厭氧條件下發(fā)酵液酶活顯著降低，0.1%TW-80對菌株Y-UA-18的產(chǎn)酶沒有影響。關(guān)鍵詞：產(chǎn)黃纖維單胞菌；纖維素酶；誘

3、變；發(fā)酵條件中圖分類號：X172文獻(xiàn)標(biāo)志碼：A文章編號：2095-6819（2016）03-0262-06doi:10.13254/j.jare.2015.0269引用格式：楊繼業(yè)，崔冠慧，習(xí)彥花，等.產(chǎn)黃纖維單胞菌CR-14菌株誘變選育及發(fā)酵條件優(yōu)化[J].農(nóng)業(yè)資源與環(huán)境學(xué)報,2016,33（3）:262-267.YANGJi-ye,CUIGuan-hui,XIYan-hua,etal.ScreeningandMutationBreedingoftheCelloulonmonasflavigenaCR-

4、14andOptimizationofItsFermentationConditions[J].JournalofAgriculturalResourcesandEnvironment,2016,33（3）:262-267.ScreeningandMutationBreedingoftheCelloulonmonasflavigenaCR-14andOptimizationofItsFermentationConditionsYANGJi-ye,CUIGuan-hui,XIYan-hua,LIYa-bin

5、g,ZHANGGen-wei,ZHANGLi-ping,CHENGHui-cai*（InstituteofBiology,HebeiAcademyofSciences,Shijiazhuang050081,China）Abstract：TheCelloulonmonasflavigenaCR-14wasscreenedfromtherumenofcattle,whichhadtheabilityofproductingcelluloseandwasstoredinourlaboratory.Inorder

6、toimprovethecelluloseactivityoftheCR-14,itwastreatedwithnitrite,ultraviolet（UV）andcomplexmutagenesis.Initialselectionidentified6cellulase-producingstrains,asdemonstratedbytheirabilitytoturnCongoredplatesyellow.These6strainswerethenrescreenedbydeterminingt

7、hecellulaseactivityofbroth.Finally,throughnitriteandUVmutagenesis,Y-UA-18strainwiththehighcelluloseyieldandstablecharacteristics,whichenzymaticactivitywas10.57U,1.67timesofstartingstrainCR-14,wasscreenedout.Inordertoprovideatheoreticalreferencetotheindust

8、rialproductionofthestrainY-UA-18,weconductedthesinglefactortestandorthogonaltesttothefermentationmediumcomponentsandthefermentationconditions.TheresultsshowedthatthebestmediumofstrainY-UA-18enzymeproductionwasasfoll