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1、林業(yè)科學(xué)研究2017,30(4):582587ForestResearchDOI:10.13275/j.cnki.lykxyj.2017.04.007黑楊應(yīng)答楊生褐盤二孢專化型侵染的基因差異表達(dá)11131,2王凱英,高茜,孫曉明,張琰鋒,嚴(yán)東輝(1.中國(guó)林業(yè)科學(xué)研究院森林生態(tài)環(huán)境與保護(hù)研究所北京100091;2.南京林業(yè)大學(xué)南方現(xiàn)代林業(yè)協(xié)同創(chuàng)新中心江蘇南京210037;3.北京林業(yè)大學(xué)林學(xué)院北京100083)摘要:[目的]楊生褐盤二孢的單芽管?;秃投嘌抗軐;头謩e引起白楊組和黑楊組(包括青楊組)楊樹的褐斑病,其分
2、化機(jī)制有待闡明。[方法]本文在?;曰プ黧w系建立的基礎(chǔ)上,利用熒光染色標(biāo)記研究了病菌孢子在?;约闹髑秩具^(guò)程中的萌發(fā)發(fā)育情況,并通過(guò)RTqPCR分析了黑楊寄主抗病相關(guān)基因在應(yīng)答兩?;颓秩具^(guò)程中的不同表達(dá)。[結(jié)果]結(jié)果發(fā)現(xiàn)多芽管分生孢子在黑楊寄主上能完成發(fā)育并成功侵染,單芽管分生孢子也能成功萌發(fā)和發(fā)育,但不能侵入黑楊寄主;黑楊抗性基因在兩專化型侵染發(fā)育過(guò)程中均能被誘導(dǎo)表達(dá),但在表達(dá)時(shí)間和表達(dá)量上,除基因WRKY89的表達(dá)較為一致外,病程相關(guān)基因PR5、PR10、NPR1和LAR3在二者間的表達(dá)存在差異。[結(jié)論]研
3、究結(jié)果初步表明病原?;涂赡苁羌闹髋c病原專性互作的表型,這一認(rèn)識(shí)將有助于進(jìn)一步探明楊樹褐斑病病原?;托纬珊头只臋C(jī)制。關(guān)鍵詞:楊生褐盤二孢,?;停遥元玻瘢校茫?,基因表達(dá)中圖分類號(hào):S796文獻(xiàn)標(biāo)識(shí)碼:A文章編號(hào):10011498(2017)04058206GeneDifferentialExpressiononPopulusdeltoidsRespondingtoInfectionbyMarssoninabrunneaFormae11131,2WANGKaiying,GAOQian,SUNXiaom
4、ing,ZHANGYanfeng,YANDonghui(1.ResearchInstituteofForestEcology,EnvironmentandProtection,ChineseAcademyofForestry,Beijing100091,China;2.CoInnovationCenterforSustainableForestryinSouthernChina,Nanjing210037,Jiangsu,China;3.ForestryCollege,BeijingForestryUnive
5、rsity,Beijing100083,China)Abstract:[Objective]TostudythemechanismofMarssoninabrunneasp.monogermtubiformaespeciales(RHBH)infectingPopulusSectionLeuceandM.brunneasp.multigermtubiformaespeciales(RHHB)infectingPopulusSectionAigeiros.[Method]Basedonpathogenhostint
6、eractionsystem,thepathogen’ssporegermination,growthandinfectionprocessonpoplarleaveswereinvestigatedbyusingfluorescencemicroscopystrainmethodandtheexpressionoffivepathogenrelativegenesinhostswasalsoanalyzedbyRTqPCRtechnique.[Result]Theresultsshowedthatthesp
7、oresofRHHBwereabletogrowandsuccessfullyinfectthehostsofPopulusSectionAigeiros,thesporesofRHBHwerealsoabletogrowbutfailedtoinfectthehostsofPopulusSectionAigeiros.ThepathogenresistantgenesofPopulusSectionAigeiroscouldbeinducedandexpressduringtheinfectionproces
8、softhetwoformaespeciales.ThegeneexpressofWRIKY89followedasimilarexpressiontrend,whiletheotherfourgenes(PR5,PR10,NPR1andLAR3)followeddifferentexpressionpatternsintimeandquantityfort