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1、摘要分子印跡技術(shù)是一項(xiàng)集高分子聚合物制備高選擇性分子識(shí)別分離富集等手段為一體的綜合性技術(shù)本論文應(yīng)用分子印跡技術(shù)以非磺脲類降血糖手性藥物那格列奈為模板分子分別采用本體聚合法原位聚合法沉淀聚合法制備了無(wú)定形連續(xù)整體和微球形具有特定識(shí)別和手性拆分性能的分子印跡聚合物用作高效液相色譜手性固定相實(shí)現(xiàn)了那格列奈分子與其對(duì)映體的手性拆分全文分為如下五個(gè)部分第一章介紹了分子印跡技術(shù)的原理研究應(yīng)用和進(jìn)展?fàn)顩r并概述了高效液相色譜及相關(guān)技術(shù)在藥物分析中的應(yīng)用第二章采用本體聚合法原位聚合法沉淀聚合法制備了不同形態(tài)那格列奈分子印跡聚合物并用作高效液相色譜手性固定
2、相考察其對(duì)那格列奈分子與其對(duì)映體的拆分色譜行為第三章考察了那格列奈印跡聚合物的吸附特性和手性分離過(guò)程中的熱力學(xué)性質(zhì)第四章應(yīng)用那格列奈分子印跡聚合物對(duì)那格列奈與其幾何異構(gòu)體進(jìn)行拆分并討論了ＭＩＰ分子識(shí)別機(jī)理同時(shí)對(duì)分子印跡技術(shù)在結(jié)構(gòu)類似物分離方面的應(yīng)用作了簡(jiǎn)單介紹第五章應(yīng)用ＨＰＬＣＥＬＳＤ技術(shù)對(duì)藥物中的膽酸植物中的水蘇糖進(jìn)行了檢測(cè)并預(yù)測(cè)了這項(xiàng)技術(shù)與分子印跡技術(shù)相結(jié)合在藥物分析中的應(yīng)用前景實(shí)驗(yàn)通過(guò)對(duì)那格列奈印跡聚合物合成手性拆分的色譜行為吸附特性和熱力學(xué)性質(zhì)等內(nèi)容的考察性和應(yīng)用優(yōu)勢(shì)驗(yàn)證了那格列奈印跡聚合物作為色譜手性固定相的可行關(guān)鍵詞分子印跡
3、聚合物高效液相色譜手性固定相那格列奈 iＡｂｓｔｒａｃｔ MolecularImprintingTechnology(MIT)hasbeenprovedtobeaneffectivetoolforchiralseparation,solid-phaseextraction,sensors,etc.Inthisthesis,molecularlyimprintedpolymers(MIP)basedonnateglinide,anorallydrugofactivesecretagogue,werepreparedandhavebeenu
4、sedasHPLCchiralstationaryphases(CSP).Thechromatographicperformance,absorptionandthermodynamiccharacteristicsofchiralseparationonMIP-CSPwereinvestigated.Therearefivechaptersinthisthesis.InChapter,theprincipleofMIT,polymerizationmethods,applicationofthistechniqueanditsfutu
5、redevelopmentswereintroduced.AndtheapplicationsofHPLConchiralseparationandHPLC-ELSDtechnologywerealsodescribed.InChapter,bulk,rodandmicrosphereMIPwerepreparedbydifferentapproaches.ExperimentsshowedthatallofthoseMIPhadwonderfulenantio-selectivitiesonnateglinideanditsL-ena
6、ntiomereventheirchromatographicperformancesweredifferent.InChapter,thecharacteristicsofadsorptionandthermodynamicsofnateglinide-MIPwereinvestigated.TheadsorptionisothermsofneteglinideandL-enantiomerwerestudiedbystaticmethod.Schatchardanalysisindicatedthattwoclassesofbind
7、ingsitewerepresentintheimprintedpolymers.InChapter,separationofneteglinideanditscis-isomeronnateglinide-MIPwasperformedandtherecognitionmechanismwasdeduced.Meanwhile,theanalogcompoundseparationsonMIPwerealsointroduced.InChapter,cholicacidinAn-GongNiuhuangcapsulesandstach
8、yoseinStachyssieboldiiMiq.weredeterminedbyHPLC-ELSD.ItwasexpectedthattheMIPcouldbeusedmorewidelybyusing
