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1、JournalofVirologicalMethods,14(1986)335-343335ElsevierJVM00538TwoenzymeimmunoassaysforthedetectionofantibodytorodentcoronavirusesAbigailL.Smith-2andDeborahF.Winogradl‘SectionofComparativeMedicine,and‘DepartmentofEpidemiologyandPublicHealth,YaleUniversitySchoolofMedicine,NewHaven,CT06510U.S.A.(Accept
2、ed18July1986)Twoenzymeimmunoassaysfordetectionofantibodytorodentcoronaviruseswerecompared.Mousehepatitisvirus(MHV),strainJHM,antigenwasintheformofformalin-fixed,infected17Cl1cells.ThisantigendetectedantibodytothehomologousstrainofMHVaswellastotwoheterologousMHVstrainsandaserologicallyrelatedratcoron
3、avirus,sialodacryodenititisvirus.Antibodytitersinassaysusinghorseradishperoxidase(HRP)-conjugatedorureiase-conjugatedanti-mouseIgGweresubstan-tiallyhigherthaninanindirectimmunofluorescenceassay.Theureiaseassaywassomewhatmoresen-sitivethantheHRPassay.MHV-JHMantigenwasstableunderavarietyofstoragecondi
4、tionsforatleasttwomonths.rodentcoronavirus,mousehepatitisvirus,sialodacryoadenitisvirus,enzymeimmunoassay,horse-radishperoxidase,ureiaseIntroductionThesentimentwasexpressedtwentyyearsagothatthecomplementfixation(CF)testwasinadequateforthedetectionofantibodytomousehepatitisvirus(MHV)amongmiceinfected
5、severalmonthspriortotesting(Parkeretal.,1966).Despitereportsofmoresensitiveserologicaltests,itwasseveralyearsbeforethesemeth-odsweregenerallyusedinrodentvirusserologylaboratories.Boththeconven-tionalenzyme-linkedimmunosorbentassay(ELISA)andindirectimunofluoresc-ence(IFA)testwereshowntobesignificantl
6、ymoresensitivethantheCFtestfordetectionofantibodytoMHV(Petersetal.,1979;Boormanetal.,1982;Smith,1983).AcommerciallyavailableELISAkitwassomewhatlesssensitivethanIFAfordetectionofMHVantibodyinseraofexperimentallyinfectedmice(Smith,1983).ThecloseantigenicrelationshipbetweenMHVandthesialodacryodenitis(S
7、DAV)ratcoronavirus(RCV)complexallowedtheuseofMHVantigenassub-stratefordetectionofantibodytotheseratcoronaviruses.TheELISAandIFAtestsusingMHVantigenweremoresensitivethantheCForneutralizationtest0166-09
