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1、PublishedOctober1,1991AGolgiRetentionSignalinaMembrane-spanningDomainofCoronavirusElProteinAnnM.SwiftandCarolynE.MachamerDepartmentofCellBiologyandAnatomy,TheJohnsHopkinsUniversitySchoolofMedicine,Baltimore,Maryland21205Abstract.TheElglycoproteinfromanaviantheirsinglemembrane-spanningdomains
2、werere-coronavirusisamodelproteinforstudyingretentionplacedwiththefirstmembrane-spanningdomainfromintheGolgicomplex.InanimalcellsexpressingtheEl.SingleaminoacidsubstitutionsinthissequenceproteinfromcDNA,theElproteinistargetedtocisreleasedretentionofthechimericGprotein,aswellGolgicisternae(Ma
3、chamer,C.E.,S.A.Mentone,asamutantElproteinwhichlacksthesecondandJ.K.Rose,andM.G.Farquhar.1990.Proc.Natl.thirdmembrane-spanningdomains.Theimportantfea-Acad.Sci.USA.87:6944-6948).Weshowthatthetureoftheretentionsequenceappearstobetheun-Downloadedfromfirstofthethreemembrane-spanningdomainsofthec
4、hargedpolarresidueswhichlineonefaceofapre-Elproteincanretaintwodifferentplasmamembranedictedalphahelix.ThisisthefirstretentionsignaltoproteinsintheGolgiregionoftransfectedcells.BothbedefinedforaresidentGolgiprotein.ThefactthatitthevesicularstomatitisvirusGproteinandthealpha-ispresentinamembr
5、ane-spanningdomainsuggestsasubunitofhumanchorionicgonadotropin(anchoredtonovelmechanismofretentioninwhichthemembranethemembranebyfusionwiththeGproteinmem-compositionoftheGolgicomplexplaysaninstrumen-brane-spanningdomainandcytoplasmictail)werere-talroleinretainingitsresidentproteins.jcb.rupre
6、ss.orgtainedintheGolgiregionoftransfectedcellswhenORTINGofnewlysynthesizedproteinsintheexocyticTheGolgicomplexplaysacentralroleintheprocessingpathwayisafundamentalproblemincellbiologywhichandsortingofnewlysynthesizedproteins(reviewedinrefer-onApril3,2015Shasreceivedagreatdealofattentioninrec
7、entyears.ence9).Itscharacteristicmorphology(stacksofflattenedSecretedandplasmamembraneproteinsfollowacommonsaccularmembranes)andcentrallocation(peri-orjuxtanu-pathwaythroughthecell:fromtheER,throughtheGolgiclear)inthecellmaybeimportantforthesefunct
