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《氧化低密度脂蛋白對(duì)人臍靜脈內(nèi)皮細(xì)胞connexin40表達(dá)的影響》由會(huì)員上傳分享，免費(fèi)在線(xiàn)閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、腦血管病防治)2010年10月第鲞箜·349··基礎(chǔ)研究·氧化低密度脂蛋白對(duì)人臍靜脈內(nèi)皮細(xì)胞Connexin40表達(dá)的影響郭士遵，章順榮，王寧夫，洪鳴嗚，高越，王曉楠，封菲(杭州市第一人民醫(yī)院，浙江杭州310006)中圖分類(lèi)號(hào)：R541．4文獻(xiàn)標(biāo)識(shí)碼：A文章編號(hào)：1009—816X(2010)05—0349—05DOI：10．3969／j．issn．1009—816X．2010．05．09TheEfectofOxidizedLDLontheExpressionofConnexin40GeneinCulturedHuma
2、nUmbilicalVeinEndotheliumCel1．GUOShi一盈m，ZHANGShun-rong，WANGNing-fu，eta1．DepartmentofCardiology，F(xiàn)irstPeople’sHospitalofHangzhou，Zhejiang310006，China[Abstract]ObjectiveToinvestigatetheefectofOxidizedLDL(Ox-LDL)ontheexpressionofGapjunctionproteinConnexin40geneincul
3、turedhumanmnbilicalveinendotheliumcell(HUVEC)．MethodsHUVECwasintervenedwithdiferentconcentrationsofOX—LDL：HUVECculturedweredividedintocontrolgroup，andOx—LDL50mg／L，lOOmg／Land200mg／Linterventiongroups，24hoursafterbeinggivenoxidizedLDL，theexpressionofConnexindOgene
4、mRNAofculturedHUVECwasdetectedwithRT-PCR；Forinter—ventionwithsameconcentrationofOX—LDLbutdiferenttime，HUVECweredividedinto6groups，theyallwereintervenedwithOX—LDL50mg／Lbutintervenedfor0hour(controlgroup)，6hours，12hours，24hours，48hoursand72hours，respectively；while
5、theexpressionlevelofConnexin40proteinwasdetectedwithimmunoeytoehemistrymethodwhileHUVECwasintervenedwithOx-LDLOmg／LandOX—LDLlOOmg／L．ResultsDiferentconcentrations(50mg／L，lOOrog／L，200mg／L)ofOX-LDLcanup-regulatemRNAexpressionlevelofConnexin40geneinculturedHUVECafte
6、r24hoursintervention(P<0．O1)．InterventionwithOx—LDL50mg／Lfordiferenttimescanalup-regulatethemRNAexpressionlevelofculturedHUVEC(P<0．01)，amongthemthemostsignificantup—regI】1ationefectwasingroupsofinterventionfor6hoursand12hours(P<0．01)．Comparedwiththecontrolgroup，
7、theproteinexpressionlevelofConnexin40geneofculturedHUVECinthegroupintervenedwithOX—LDL100m#Lfor24hourswasmarkedlyup-regulated(P<0．01)．ConclusionsOx—LDLmayup-regulatetheproteinandmRNAexpressionlevelofConnexin40geneincul—turedHUVECwithinshor【time，indicatingthatCon
8、nexin40mayplayanimportantroleinthepro-atheroscleroticefectofOX—LDL．[KeywordsJGapjunctionprotein；Connexin40；OxidizedLDL；HumanveinendotheliumeeH；Geneexpression動(dòng)脈粥樣硬化(At
