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《氧化低密度脂蛋白對人臍靜脈內皮細胞connexin40表達的影響》由會員上傳分享，免費在線閱讀，更多相關內容在學術論文-天天文庫。
1、腦血管病防治)2010年10月第鲞箜·349··基礎研究·氧化低密度脂蛋白對人臍靜脈內皮細胞Connexin40表達的影響郭士遵，章順榮，王寧夫，洪鳴嗚，高越，王曉楠，封菲(杭州市第一人民醫(yī)院，浙江杭州310006)中圖分類號：R541．4文獻標識碼：A文章編號：1009—816X(2010)05—0349—05DOI：10．3969／j．issn．1009—816X．2010．05．09TheEfectofOxidizedLDLontheExpressionofConnexin40GeneinCulturedHuma
2、nUmbilicalVeinEndotheliumCel1．GUOShi一盈m，ZHANGShun-rong，WANGNing-fu，eta1．DepartmentofCardiology，F(xiàn)irstPeople’sHospitalofHangzhou，Zhejiang310006，China[Abstract]ObjectiveToinvestigatetheefectofOxidizedLDL(Ox-LDL)ontheexpressionofGapjunctionproteinConnexin40geneincul
3、turedhumanmnbilicalveinendotheliumcell(HUVEC)．MethodsHUVECwasintervenedwithdiferentconcentrationsofOX—LDL：HUVECculturedweredividedintocontrolgroup，andOx—LDL50mg／L，lOOmg／Land200mg／Linterventiongroups，24hoursafterbeinggivenoxidizedLDL，theexpressionofConnexindOgene
4、mRNAofculturedHUVECwasdetectedwithRT-PCR；Forinter—ventionwithsameconcentrationofOX—LDLbutdiferenttime，HUVECweredividedinto6groups，theyallwereintervenedwithOX—LDL50mg／Lbutintervenedfor0hour(controlgroup)，6hours，12hours，24hours，48hoursand72hours，respectively；while
5、theexpressionlevelofConnexin40proteinwasdetectedwithimmunoeytoehemistrymethodwhileHUVECwasintervenedwithOx-LDLOmg／LandOX—LDLlOOmg／L．ResultsDiferentconcentrations(50mg／L，lOOrog／L，200mg／L)ofOX-LDLcanup-regulatemRNAexpressionlevelofConnexin40geneinculturedHUVECafte
6、r24hoursintervention(P<0．O1)．InterventionwithOx—LDL50mg／Lfordiferenttimescanalup-regulatethemRNAexpressionlevelofculturedHUVEC(P<0．01)，amongthemthemostsignificantup—regI】1ationefectwasingroupsofinterventionfor6hoursand12hours(P<0．01)．Comparedwiththecontrolgroup，
7、theproteinexpressionlevelofConnexin40geneofculturedHUVECinthegroupintervenedwithOX—LDL100m#Lfor24hourswasmarkedlyup-regulated(P<0．01)．ConclusionsOx—LDLmayup-regulatetheproteinandmRNAexpressionlevelofConnexin40geneincul—turedHUVECwithinshor【time，indicatingthatCon
8、nexin40mayplayanimportantroleinthepro-atheroscleroticefectofOX—LDL．[KeywordsJGapjunctionprotein；Connexin40；OxidizedLDL；HumanveinendotheliumeeH；Geneexpression動脈粥樣硬化(At
