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1、1O6食品與藥品FoodandDrug2014年第16卷第2期薯莨色素的提取工藝及基本成分和抗菌活性研究彭建飛,范潤(rùn)珍,李曉菲(1.深圳市計(jì)量質(zhì)量檢測(cè)研究院,廣東深圳518109;2.廣東海洋大學(xué)理學(xué)院,廣東湛江524025;3.湛江市農(nóng)業(yè)技術(shù)推廣中心,廣東湛江524038)摘要:目的探討薯莨色素的提取工藝,研究其基本成分和抗菌活性。方法以60%丙酮為提取劑,料液比(w/v)1:1o,常溫浸漬5h,60℃水浴回流3h,提取薯莨色素;測(cè)定其水分、灰分、粗蛋白、粗脂肪及粗纖維等含量;并以大腸桿菌、桉樹(shù)青菇菌、巴氏桿菌、蠟狀芽孢桿菌、芽孢桿菌、沙門(mén)菌為供試菌,檢驗(yàn)提取薯莨色素的抗菌活性。結(jié)
2、果薯莨色素提取率達(dá)28.6%;薯莨色素基本成分為:水分22.61%,灰分1.61%,粗脂肪2.89%,粗纖維2.80%,粗蛋白3.80%;薯莨色素濃度在0.1%~0.50%范圍內(nèi)對(duì)6種供試菌種皆顯示了不同程度的抑制效果。結(jié)論薯莨色素是具有抗菌活性的天然色素,具有一定的開(kāi)發(fā)價(jià)值。關(guān)鍵詞:薯莨;色素;提取工藝;抑菌活性中圖分類(lèi)號(hào):11284.1文獻(xiàn)標(biāo)識(shí)碼:A文章編號(hào):1672.979X(2014)02.0106.04ResearchonExtractionTechnologyofDioscoreaCirrhosaPigmentandItsElementaryComponentsandAn
3、tibacterialandAntifungalActivityPENGJian.fei,F(xiàn)ANRun.zhen,LIXiaofei(1.ShenzhenAcademyofMetrology&QualityInspection,Shenzhen518109,China;2.CollegeofScienceofGuangdongOceanUnivercity,Zhanjiang524025,China;3.AgricultureTechnologyPopularizingCenterinZhanj’iangZhanjiang524038,China)Abstract:Objective
4、ToexploretheextractiontechnologyofDioscoreacirrhosapigmentandtoresearchtheelementarycomponentsandantibacterialactivityoftheextractedpigment.MethodsTheDiosoreacirrhosapigmentwasextractedunderthecondition:60%acetonewasusedasextractantwiththesolid.1iquidratio1:10.thesamplewassoakedfor5hatnormaltem
5、perature.thenwasrefluxedfor3hinawaterbathat60℃.TheantibacterialactivityoftheDioscoreacirrhosapigmentonEscherichiacoli,Eucalyptusgreenmushroomfungus,Pasteurellamultocide,Bacilluscereus,GemmabacillusandSalmonellawastested.Thecontentsofelementarycomponentsofthepigmentincludingmoisture,ash,crudefat
6、,crudefiberandcrudeproteinweredetermined.ResultsTheextractionrateofDiosoreacirrhosapigmentreached28.6%.Thecontentsofelementarycomponentswereasfollows:moisture22.61%.a(chǎn)sh1.61%,crudefat2.89%,crudefiber2.80%andcrudeprotein3.80%.Intheconcentrationrangeof0.1%-0.50%.thepigmentshoweddiferentdegreeofinh
7、ibitoryefectsonthegrowthoftheabovebacteriaandfungus.ConclusionTheDiosoreacirrhosapigmentwasakindofnaturalpigmentwithantibacterialandantifungalactivityandhasdevelopmentalvalueasnaturaldye.KeyWords:Dioscoreacirrhosa;pigment;extracti