轉(zhuǎn)染 PDCD5質(zhì)粒促進(jìn)順鉑誘導(dǎo) A549細(xì)胞凋亡作用的研究-論文.pdf

《轉(zhuǎn)染 PDCD5質(zhì)粒促進(jìn)順鉑誘導(dǎo) A549細(xì)胞凋亡作用的研究-論文.pdf》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在行業(yè)資料-天天文庫。

1、重慶醫(yī)學(xué)2014年9月第43卷第26期3449論著·基礎(chǔ)研究doi：10．3969／j．issn．1671—8348．2014．26．015轉(zhuǎn)染PDCD5質(zhì)粒促進(jìn)順鉑誘導(dǎo)A549細(xì)胞凋亡作用的研究戴玲麗，梅同華，張明川。(1．南昌大學(xué)第一附屬醫(yī)院呼吸內(nèi)科，南昌330006；2．重慶市銅梁縣人民醫(yī)院呼吸內(nèi)科402560)摘要：目的觀察程序化死亡因子5(PDCD5)基因聯(lián)合順鉑誘導(dǎo)人肺腺癌A549細(xì)胞凋亡的作用，探索其可能機(jī)制。方法利用脂質(zhì)體瞬時(shí)轉(zhuǎn)染PDCD5重組質(zhì)粒入A549細(xì)胞，RT-PCR檢測(cè)其轉(zhuǎn)染效率。Westernblot檢測(cè)轉(zhuǎn)染后PDCD5、Bcl-2和Survivin蛋白表達(dá)情況。

2、MTT法及流式細(xì)胞儀檢測(cè)PDCD5單藥和聯(lián)合順鉑后的細(xì)胞凋亡情況。結(jié)果PDCD5重組質(zhì)粒成功轉(zhuǎn)染入A549細(xì)胞，Westernbolt結(jié)果顯示轉(zhuǎn)染重組質(zhì)粒組中PDCD5蛋白表達(dá)高于空白對(duì)照組及轉(zhuǎn)染空質(zhì)粒組，Bcl一2和Survivin蛋白則低于空白對(duì)照組及轉(zhuǎn)染空質(zhì)粒組，差異有統(tǒng)計(jì)學(xué)意義(P<0．05)。MTT及流式細(xì)胞儀檢測(cè)顯示轉(zhuǎn)染重組質(zhì)粒組較空白對(duì)照組及轉(zhuǎn)染空質(zhì)粒組細(xì)胞凋亡率高(P

3、4)26-3449-03TransfectionofPDCD5plasmidforpromotingcisplatin-inducedA549cellsapoptosisDaiLingli，MeiTqnghua1ix，ZhangMingchuan。(1．DepartmentofRespiration，F(xiàn)irstA，liatedHospital，SchoolofMedicine，NanchangUniversity，Nanchang，Jiangxi330006，China；2．DepartmentofRespiration，TongliangCountyPeoplesHospital，Chon

4、gqing402560，China)Abstract：ObjectiveToobservetheroleofprogrammedcelldeath5(PDCD5)genecombinedwithcisplatinforinducingtheap—optosisofhumanlungadenocarcinomaA549cellsandtoinvestigateitspossiblemechanism．MethodsThePDCD5recombinantplas—midwastransientlytransfeetedintoA549cellsbylipofectamine．Itstransfe

5、ctionefficiencywasdetectedbyRT-PCR．TheexpressionsoftrasfectedPDCD5protein．Bcl-2andSurvivinproteinwereexaminedbyWesternbolt．TheapoptosisofA549cellsbysinglePDCD5anditscombinationwithcisplatinwasmeasuredbytheMTTmethodandtheflowcytometry．ResultsPDCD5recombinantplasmidwastransfectintoA549cellssuccessful

6、ly．TheWesternblotresultsshowedthattheexpressionofPDCD5proteininthetransfectionrecombinantplasmidgroupwashigherthanthatintheblankcontrolgroupandthetransfectionemptyplasmidgroup，whiletheexpres—sionofBcl_2andSurvivinproteinwaslowerthanthatintheothertwogroups，thedifferencewasstatisticallysignificant(P<

7、0．05)．TheMTTandflowcytometryresultsdemonstratedthatthecellapoptosisrateinthetransfectionrecombinantplasmidgroupwashigherthanthatintheblankcontrolgroupandthetransfectionemptyplasmidgroup(P<0．05)．ConclusionPD