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1、世界中西醫(yī)結(jié)合雜志2014年第9卷第8期WorldJoumalofIntegratedTraditionalandWesternMedicine2014．Vo1．9No8·829·實驗研究·濕熱瘀阻型類風濕關(guān)節(jié)炎外周血單個核細胞GPImRNA水平的研究馮雪姜泉安成陳倩云邱紅梅劉貴建【摘要】目的探討濕熱瘀阻型類風濕關(guān)節(jié)炎(Rheumatoidarthritis，RA)外周血單個核細胞葡萄糖6一磷酸異構(gòu)酶(GPI)水平與其他證型類風濕關(guān)節(jié)炎及正常人群之間的關(guān)系。方法采用熒光定量PCR方法檢測GPImRNA水平，研究對象包括RA患者47例，其中濕熱瘀阻型27例，
2、痰瘀互結(jié)型20例，其他風濕病患者對照組20例，3O名健康體檢者。結(jié)果RA濕熱瘀阻組GPImRNA的相對表達量是健康對照組的25．9倍，差異有統(tǒng)計學意義(P<0．05)。RA痰瘀互結(jié)組GPImRNA的相對表達量是健康對照組的6．54倍，而RA濕熱瘀阻組與RA痰瘀互結(jié)組差異也有統(tǒng)計學意義(P<0．05)。其他風濕病組GPImRNA的相對表達量是健康對照組的0．97倍，差異無統(tǒng)計學意義(P>0．05)。結(jié)論GPI與RA活動性有關(guān)，同時證明濕熱瘀阻證是RA活動期的證候之一?！娟P(guān)鍵詞】葡萄糖一6～磷酸異構(gòu)酶；類風濕關(guān)節(jié)炎；逆轉(zhuǎn)錄一聚合酶鏈反應(yīng)【中圖分類號】R593．
3、22【文獻標識碼】A【doi】10．13935／j．cnki．sjzx．140811StudyonGPImRNALevelinPerioheralBloodMononuclearCellinRheu．matoidArthritisofDamp．．Heat．．StasisObstructionPatternFENGXue，JIANGQnan，ANCheng，CHENQian—yun，QIUHong—mei，LIUGui—jian(GuanganmenHospitalofChinaAcademyofChineseMedicalSciences，Beijing1
4、00053)【Abstract】ObjectiveToexploretheglucose6一phosphateisomerase(GPI)levelinperipheralbloodmononuclearceilsinrheumatoidarthritis(RA)ofdamp—heat—stasisobstructionpatternaswellastherelationshipbetweenRAdifferentiatedpatternandnormalgroup．MethodsThefluorescencequantitativePCRmethodwa
5、susedtodetectthelevelofGPImRNA．Thesubjectsincluded47RApatients，including27casesweredifferentiatedasdamp—heat—stasisobstructionand20casesofinteractionofphlegmandstasis．Additionally，20casesofotherrheumatoiddiseaseswereinacontrolgroupand30casesinanormalgroup．ResultsInRAdamp—heat—stas
6、isstagnationgroup，therelevantexpressionofGPImRNAwas25．9timeshigherthanthatinthenormalgroup，indicatingthestatisticalsignificantdifference(P<0．05)．TherelevantexpressionofGPImRNAinRAphlegm—stasisinteractiongroupwas6．54timeshigherthanthatinthenor—realgroup．Thedifferencewassignificantb
7、etweenRAdamp—heat—stasisobstructiongroupandRAphlegm—stasisinteractiongroup(P<0．05)．TheexpressionofGPImRNAintherheumatoiddiseasegroupwas0．97timeshigherthanthatinthenormalgroup，withoutsignificantdifference(P>0．05)．ConclusionGPIisrelatedtoRAactivity．Itisprovedthatdamp—heat—stasisobst
8、ructionsyndromeisoneofthedisorder
