資源描述:
《擬南芥木聚糖合成關(guān)鍵酶基因的調(diào)控研究》由會員上傳分享,免費在線閱讀,更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、華南農(nóng)業(yè)大學(xué)學(xué)報2014,35(4):97-102http:∥xuebao.scau.edu.cnJournalofSouthChinaAgriculturalUniversitydoi:10.7671/j.issn.1001-411X.2014.04.018王玉琪,賀俊波,吳藹民.?dāng)M南芥木聚糖合成關(guān)鍵酶基因的調(diào)控研究[J].華南農(nóng)業(yè)大學(xué)學(xué)報,2014,35(4):97-102.?dāng)M南芥木聚糖合成關(guān)鍵酶基因的調(diào)控研究112王玉琪,賀俊波,吳藹民(1華南農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院,廣東廣州510642;2華南農(nóng)業(yè)大學(xué)新能源與新材料研究所,廣東廣州510642)摘要:【目的】從目前已知的參
2、與擬南芥Arabidopsisthaliana次生壁加厚生長的轉(zhuǎn)錄因子著手,分析這些次生壁相關(guān)的轉(zhuǎn)錄因子是否能夠調(diào)控木糖合成關(guān)鍵酶基因FRA8、IRX9、IRX10、IRX14、F8H、IRX9-L、IRX10-L和IRX14-L的表達,并且觀察KNAT7基因顯性抑制植株的表型.【方法】通過Gateway技術(shù)構(gòu)建效應(yīng)器和報告器,進行瞬時轉(zhuǎn)錄激活試驗,同樣構(gòu)建pCAMBIA1304-p35S∷KNAT7-SRDX重組質(zhì)粒,用農(nóng)桿菌Agrobacteriumtumefaciens花序浸染法將此質(zhì)粒轉(zhuǎn)化到野生型擬南芥植株中.【結(jié)果和結(jié)論】瞬時轉(zhuǎn)錄激活試驗表明,轉(zhuǎn)錄因子KNAT7、MYB4
3、6、ERF72、SND1、NST2能夠激活多個擬南芥木聚糖合成關(guān)鍵酶基因的表達,其中KNAT7能促進基因FRA8、IRX9和IRX14-L的表達.KNAT7基因顯性抑制能顯著影響擬南芥的生長.試驗結(jié)果表明KNAT7基因可能在木聚糖的合成中起著重要的調(diào)控作用.關(guān)鍵詞:木聚糖;木聚糖合成關(guān)鍵酶基因;調(diào)控;KNAT7中圖分類號:S852.65文獻標(biāo)志碼:A文章編號:1001-411X(2014)04-0097-06StudiesontheregulationofxylanbiosynthesiskeyenzymegenesinArabidopsisthaliana112WANGYuq
4、i,HEJunbo,WUAimin(1CollegeofLifeSciences,SouthChinaAgriculturalUniversity,Guangzhou510642,China;2InstituteofNewEnergyandNewMaterials,SouthChinaAgriculturalUniversity,Guangzhou510642,China)Abstract:【Objective】ToanalyzewhethersometranscriptionfactorsinArabidopsisthaliana,knownforthesecondarycell
5、wallthicken,couldregulatetheexpressionofthekeygenesofxylosyltransferase,suchasFRA8,IRX9,IRX10,IRX14,F(xiàn)8H,IRX9-L,IRX10-LandIRX14-L,andobservethephenotypeofKNAT7dominantrepressionplant.【Method】EffectorsandreporterswereconstructedbyGatewayTech-nologyandthetransienttranscriptionalactivationassaywas
6、conducted.ConstructpCAMBIA1304-p35S∷KNAT7-SRDXrecombinantplasmidbyGatewayTechnologyandtransformthisplasmidintowildA.thali-anaviaAgrobacteriumtumefaciens-floraldipmethod.【Resultandconclusion】Thetransienttranscription-alactivationassayrevealedthattranscriptionfactorsKNAT7,MYB46,ERF72,SND1,NST2co
7、uldacti-vatetheexpressionofanumberofthekeygenesofxylosyltransferase.KNAT7couldactivatetheexpres-sionofFRA8,IRX9andIRX14-L.Furthermore,dominantrepressionofKNAT7significantlyaffectedthegrowthofArabidopsisthaliana.TheseresultsindicatethatK