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1、基質(zhì)細胞衍生因子1在骨髓間質(zhì)干細胞歸巢到急性梗死心肌中的作用2007年6月第33卷第6期Jian~suMedJ,June007'13310.基質(zhì)細胞衍生因子1在骨髓間質(zhì)干細胞歸巢到急性梗死心肌中的作用莊瑜陳鑫石開虎徐明【摘要】目的探討基質(zhì)細胞衍生因子1(SDF_1)在骨髓問質(zhì)干細胞(MSCs)歸巢到急性梗死心肌中的作用.方法以免疫組化檢測SDF-1在心肌梗死1,2,4,7,14,28d后的大鼠及非心肌梗死大鼠心肌中的表達差異;于心肌梗死4d后予以SDF-1,抗SDF-1抗體進行干預,同時經(jīng)尾靜脈注射BrdU標記的MSCs,3d后檢測
2、梗死區(qū)MSCs的歸巢量,28d后檢測大鼠心功能狀況,以及梗死區(qū)及其周圍的血管密度;另設心肌梗死及非心肌梗死陰性對照組.結果SDF-1在心肌梗死后第1天即升高并達到峰值,以后逐漸下降,第14天恢復至正常水平.心肌梗死組干細胞歸巢量明顯大于非心肌梗死對照組.SDF-1處理組大鼠心肌梗死區(qū)干細胞歸巢量,心功能水平及血管密度大于抗SDF-1抗體處理組及心肌梗死對照組(P<O.01).結論心肌梗死后早期梗死區(qū)SDF-1表達水平升高;SDF-1能夠促進MSCs歸巢并改善心功能,促進毛細血管增生可能為其改善心功能的機制之一.【關鍵詞】心肌梗
3、死;骨髓間質(zhì)干細胞;歸巢;心功能;基質(zhì)細胞衍生因子一1【中圖分類號1R541【文獻標識碼】A【文章編號10253—3685(2007)06—0579—04TheefofsI,F-1onmy瑚IsteIIlIlsl10IIliI-gtomyocardialin瞄siteZNGy",CttF_,NXin,SHlKaihu,eta1.DepartmentofThoracic&CardiovascularSurgery,AffiliatedNanjingFirstHospital,Nan]ingMedicalUniversity,N
4、aing210006,CHINA[AStract]ObjectiveToinvestigatetheeffectofstromalcell-derivedfactor-1(SDF-1)onmesenchymalstemcells(MSCs)homingtomyocardialinfarctsiteMethodsSDF-1expressionwasmeasuredbyimmunohistochemistryinshamoperatedorinfarctedheartsonthe1st,2nd,4th.7th,14thand28thda
5、yaftermyocardialinfarction(MI).MSCsfromdonorratswere1abeledwithBrdU.)F_1,anti-SDF-1antibodyorsalinewasinjectedintoperi-infarctmyocardium4daysafterMI.Then.atotalof5×10cellsin2.5m10fPBSorequalvolumePBSalonewereinjectedthroughthetai1veimThenumberoflabeledMSCsintheinfarcte
6、dheartswascotmted3daysafterinjection.Cardiacfunctionandbloodvesseldensitywereassessed28daysafterinjection.ResultsMyocardialS】)F_1expressionincreasedandpeakedonthefirstdayafterM1anddecreasedthereafter.whichremainedunchangedinshamoperatedhearts.TheMSCsinthehostheartswere
7、moreabundantintheMIgroupsthanthoseinthenon-MIgroup.whichweremoreintheSDF.1injectedgroupthanthoseintheanti-sDF-1antibodyandsalineiniectedgroups(P<0.05).CardiacfunctionwasimprovedmoreinSDF-1injectedgroupthanthatinanti—SDF-1antibodyandsalineinjectedgroups(P<0.01).Ne
8、ovascularizationinSDF-iectedgroupsignificantlyincreasedthanthatintheothergroups(P<0.01).ConclusionMyocardialSDF-1e
