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《產(chǎn)幾丁質酶細菌的鑒定與產(chǎn)酶和酶學性質的.研究》由會員上傳分享，免費在線閱讀，更多相關內容在應用文檔-天天文庫。
1、四川大學碩t論文studieswillberesearchedontheisolationandscreeningofunknownchitinaseformingstrains．Also，thechitinasefrommicrobesmustbepurified，itscharacteristics，thewayitworksandtheeffectiveitproducesarethehotspotswhichresearchedOilbiocontroltoday．Thesubjectinourexperimentisatypicalinsect(Bomb
2、yxmori)，whichdiednaturally．Outwardly，thedeadbodyhadabigbrightcoeruleusspotontheircephalothorax．Achitinaseproductivestrainwasisolatedfromthenaturallydeadbody’Speritrophicmembraneinmidgut．Themaincompositionofperitrophicmembraneischitins，itisthefirstbarrierandplaysanimportantroleinprotec
3、tingthegutepitheliumfrominfectionofpathogens．Ifthisplaceisdegradated．theremustbesomethingwhichcanhydrolyzechitins．That’Swhywechosethisplacetoisolatethechitinaseproductivemicrobes．Inordertoenrichthechitinaseproductivemicrobeswhenweculturethemixturesampleonthescreeningmediumflatplate，we
4、usedcolloidalchitinastheonlycarbonsource．Doingthis，mostmicrobeswhichcan’tusechitinasitscarbonsourceareknockedout，leftonlythemicrobeswhichcanusechitinasitscarbonsource．Duringthesemicrobes，mostcanproducechitinase．Underasepsiscondition，wetakethesamplefromthedeadbody’Speritrophicmembranei
5、nmidgut，thenseparatedtheminspacebydrawlinesonthechitinflatplate．Theplateswereincubatedat28。Cfor72h．Mostmicrobesontheplatehashydrolyticcircle，whichcausedbythechitindegradation．Takenotetotheratioofthediameterofthehydrolyticcircletothediameterofthecolony．Bycomparison，wechoseahighyielding
6、chitinase-productingstrain，namedzll-r．Thisstrainisincubatedunderthesameconditionforfivegeneration，andrecordtheratioofthediameterofthehydrolyticcircletothediameterofthecolony．Fromthisresult，wecandiscoverthatthestrainzll-rhaschitinase-productinggeneticstability．Themethodtoconservethestr
7、ainisglycerolquickfreezingmethodandpetrolineslanttubemethod．Inordertoconformthefunctionofthisstraininbiocontrol，wedeterminatedthetoxicityofthestrainzll·rtotheBombyxmori，grasshoppersandcabbagebutterflylarva．ApurestrainisseparatedfromtheLBculture．ThenweinoculatedthepureVl四川人學碩士論文strainI
8、ntoth
