資源描述：
《Development and Evaluation of a Novel Solution, Somahfor the Procurement and Preservation of Beating and,一種新型溶液SOMAH的開發(fā)和評價 關(guān)于打漿的采購和保存 非搏動供體心臟移植》由會員上傳分享，免費在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、TransplantationDevelopmentandEvaluationofaNovelSolution,Somah,fortheProcurementandPreservationofBeatingandNonbeatingDonorHeartsforTransplantationHemantS.Thatte,MSc,PhD;LakiRousou,MS,MD;BaderE.Hussaini,MD,MPH;Xiu-GuiLu,MD;PatrickR.Treanor,CCP,LCP;ShukriF.Khuri,MD?Background—Injurytomyocytes,
2、endocardium,andthecoronaryendotheliumduringharvestingandstoragecancompromiseoutcomesafterhearttransplantation.Safeguardingofstructureandfunctionofcardiomyocytesandendotheliumindonorheartsmayleadtoimprovedpatientsurvivalaftertransplantation.Informationgainedfromporcineheartsstoredinstandardt
3、ransplantsolutionwasusedtodesignasuperiorpreservationsolutionthatwouldoptimallyprotectandmaintainorgansfrombeatingheartand/ornonbeatingheartdonorsduringlong-termstorage.MethodsandResults—Multiphotonmicroscopywasusedtoimagedeepwithincardiacbiopsiesandcoronaryarterytissueharvestedfromporcineh
4、eartsobtainedfrombeatingheartandnonbeatingheartdonorsforanalysisofmyocyteandendothelialcellstructureandfunction.Cellstructuralintegrityandviability,calciummobilization,andnitricoxidegenerationweredeterminedwithfluorescenceviabilitymarkers,immunofluorescence,andWesternblots.Duringhypothermic
5、storageinstandardpreservationsolution,Celsior,myocyte,andendothelialviabilitywasmarkedlyattenuatedinheartsobtainedfrombeatingheartdonors.Incontrast,heartsfrombeatingandnonbeatingheartdonorsstoredinthenewlyformulatedSomahsolutiondemonstratedanincreaseinhigh-energyphosphatelevels,protectionof
6、cardiacmyocyteviability,mitochondrialmembranepolarization,andstructuralproteins.Similarly,coronaryarteryendothelialorganizationandfunction,calciummobilization,andnitricoxidegenerationwerewellmaintainedduringtemporalstorageinSomah.Conclusions—TheCelsiorpreservationsolutioninclinicalusetodayh
7、asledtoaprofounddeclineincardiomyocyteandendothelialcellviability,whereasthenewlydesignedSomahsolutionhassafeguardedmyocyteandendothelialintegrityDownloadedfromhttp://ahajournals.orgbyonAugust11,2018andfunctionduringorganstorage.UseofSomahasastoragemediu
