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1、腺昔預處理對糖氧剝奪損傷星形膠質(zhì)細胞的保護作用摘要：目的：觀察糖氧剝奪對星形膠質(zhì)細胞的影響及腺昔的保護作用。方法：取體外培養(yǎng)第三代或第四代星形膠質(zhì)細胞，傳代至96孔板或24孔板內(nèi)，細胞貼壁并長出突起后，隨機分為三組：正常對照組(normalcontrolgro叩)、糖氧剝奪組(OGDgroup)s腺昔預處理組(ADOgroup),進行糖氧剝奪/復氧處理。觀察糖氧剝奪8h復氧糖24h后星形膠質(zhì)細胞形態(tài)、細胞活性。結果：糖氧剝奪復氧糖24h后，正常對照組星形膠質(zhì)細胞生長狀態(tài)良好;OGD模型組星形膠質(zhì)細胞胞體水腫變圓，細胞形
2、態(tài)由不規(guī)則變成梭形，突起明顯變短或消失；ADO組細胞水腫較輕。OGD模型組星形膠質(zhì)細胞活力下降，OD值明顯低于正常對照組(P<0.01)；ADO組細胞活力顯著高于OGD模型組，表明ADO能明顯改善細胞活力(P<0.01)o結論：糖氧剝奪可引起星形膠質(zhì)細胞嚴重損傷,星形膠質(zhì)細胞形態(tài)、活性與缺氧缺糖呈對應變化，腺昔能減少糖氧剝奪后星形膠質(zhì)細胞形態(tài)學改變及細胞凋亡，提示腺昔對糖氧剝奪的星形膠質(zhì)細胞有一定的保護作用。關鍵詞：腺昔；星形膠質(zhì)細胞；糖氧剝奪TheprotectionofAdenosinePreconditionin
3、gonOxygen-glucosedeprivationinjuryinducedastrocytecells[Abstract]ObjectiveToinvestigatetheeffectofoxygen-glucosedeprivationandprotectiveeffectofadenosineonastrocytes.MethodsAstrocyteswereculturedforthirdorfourthgenerationinvitro,andthenpassagedat96or24wellplate.
4、Whentheastrocytesattachingtothewallandgrowingwithprominences,theywererandomlydividedinto3groups,normalcontrolgroup,oxygen-glucosedeprivation(OGD)groupandadenosinepreconditioning(ADO)group.ThecellsweretakenOGD/reoxygenationtreatment.Themorphologyandactivityofastr
5、ocytecellswereobservedat8hoursafterOGDand24hoursafterreoxygenation.ResultsAt24hoursafterOGD/reoxygenationtreatment,theastrocytecellswerewithnormalmorphologyinnormalcontrolgroup；thesomaofastrocytecellsbecameedemaandroundinOGDgroup,thecellmorphologychangedtofusifo
6、rmisfromirregular,andprominenceobviouslybecameshortordisappeared；theedemawaslighterinADOgroup?ActivityofastrocytecellsdecreasedinOGDgroup,andODvaluewassignificantlylowerthanthatinnormalcontrolgroup(P<0.01).CellactivitywashigherinADOgroupthanthatinOGDgroup,whichi
7、ndicatedthatADOcanobviouslyimprovedcellactivity(P<0.01)?ConclusionsAstrocytesareinjuredseriouslyafterOGD；morphologyandcellactivityofastrocytestakechangeinaccordancewithoxygendeficitandglucosedeprivation.Adenosinecandecreasemorphologicalchangesandcellapoptosisofa
8、strocytecellsafterOGD.wecanmakeconclusionthatadenosinehasinfluenceonastrocytesafterOGD.【Keywords]Adenosine；Astrocyte；Oxygen-glucosedeprivation【中圖分類號】Q2-09【文獻標識碼】B【文章編
