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1、天然產(chǎn)物研究與開(kāi)發(fā)NatProdResDev2013。25：960-962，1010文章編號(hào)：1001-6880(2013)7-0960-04不同生長(zhǎng)階段家蠶1．脫氧野尻霉素富集與食桑量的相關(guān)性研究劉月琴，歐陽(yáng)臻h，趙明，陳紅霞，潘剛，段金廒江蘇大學(xué)藥學(xué)院，鎮(zhèn)江212013；國(guó)農(nóng)業(yè)科學(xué)院蠶業(yè)研究所，鎮(zhèn)江212018；。南京中醫(yī)藥大學(xué)藥學(xué)院，南京210046摘要：為了研究家蠶富集1-脫氧野尻霉素(DNJ)的機(jī)制，采用柱前衍生化高效液相色譜法分析不同生長(zhǎng)階段家蠶及其代謝產(chǎn)物和所食桑葉中DNJ的含量，研究家蠶幼蟲(chóng)體1．脫氧野
2、尻霉素富集與食桑量的相關(guān)性。結(jié)果顯示不同生長(zhǎng)期家蠶及其副產(chǎn)物中DNJ的累積量有較大差異，且呈一定規(guī)律性變化，先上升后下降，于四齡時(shí)最高。家蠶幼蟲(chóng)期蠶體及其副產(chǎn)物中DNJ總累積量與其在該階段所食桑葉中DNJ總量相當(dāng)，且家蠶的任一成長(zhǎng)發(fā)育階段(蟻蠶和熟蠶不吃桑葉除外)，其蠶體DNJ含量均大大高于桑葉中的DNJ含量，表明家蠶具有累積或富集DNJ的機(jī)能，本研究結(jié)果為進(jìn)一步研究家蠶富集DNJ的機(jī)制提供了理論依據(jù)。關(guān)鍵詞：桑葉；家蠶；I．脫氧野尻霉素；富集；相關(guān)性中圖分類號(hào)：$88文獻(xiàn)標(biāo)識(shí)碼：ACorrelationalStudy
3、on1一DeoxynojirimycinContentinMulberryLeavesandItsEnrichmentinDiferentGrowthStageofSilkwormLarva，BombyxmoriLIUYue—qin，OUYANGZhen，ZHAOMing，CHENHong—xia，PANGang，DUANJin．a(chǎn)oSchoolPm口cy，Jiangsu，Zhenfiang212013，China；TheSericalturalResearchInstitute，ChineseAcademyofAgr
4、iculturalSciences，Zhenjiang2120183；3NanjingUniversityofChineseMedicine，Nanjing210046，ChinaAbstract：Theobjectiveofthisstudywastoinvestigatetheenrichmentmechanismofthe1-deoxynojirimycin(DNJ)inthelarvaeofthesilkworm(Bombyxmo~i)．1~re·columnderivafizafioncoupledwithr
5、eversed—phasehishperformanceliq-uidchromatography(HPLC)WasemployedtodeterminetheDNJcontentindiferentgrowthstageofsilkwormlarvaanditsbyproductsasweI1asthecontentinmulberryleaves．ThecorrelationbetweentheconsumptionofmulberryleavesandtheenrichmentofDNJinthelarvaoft
6、hesilkwormWasthenanalyzed．TheresultsshowedthattheaccumulationofDNJinBombyxmoriandtheirbyproductsindiferentgrowthperiodswereremarkablydiferent，andchangedregularly，whichWasrisingfirstandthentodechne，theaccumulationreachedthepeakatthe4thinstar．TheDNJcontentofsilkwo
7、rmbodyandits’byproductsWasroughlythesglneasthecontentinmulberryleavesthatsilkwormate．TheDNJcontentofsilkwormWasmuchhigherthantheDNJcontentofmulberryleavesindiferentgrowthstageofsilkwormlarva．Thisstudyrevealedthatthesilkwormhadthefunctionofenrichingandaccumulatin
8、gDNJ．TheresulmprovidedthebasisforfurtherresearchofstudyingfheDNJenrichmentandmetabolisminthelar'caeofthesilkworm．Keywords：mulberryleaf；Bombyxmor／；1-deoxynojirimycin；e
