槐米中蘆丁超聲法和堿溶酸沉法提取比較畢業(yè)論文

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1、2011屆　　　　　　　　　分類(lèi)號(hào)：　　　　　　　　　　　　　　　　　單位代碼：10452畢業(yè)論文槐米中蘆丁超聲法和堿溶酸沉法提取比較ComparationofUltrasonicandAlkalisolutionandacidprecipitationextractedRutinfromSophorajaponica·1摘要槐花中主要成分為蘆丁,又名蕓香苷,現(xiàn)在報(bào)道的提取方法有很多，本實(shí)驗(yàn)主要研究超聲提取法與堿溶酸沉法,比較這兩種方法提取率高低。超聲法是用70%乙醇溶液浸泡槐米粉，在超聲清洗器

2、中進(jìn)行超聲處理30min，處理2次，收集濾液，用濃鹽酸調(diào)節(jié)PH值至3，靜置，至沉淀完全后，抽濾，收集沉淀得到蘆丁粗品，粗品在60-70℃水浴中溶解，煮沸15min，趁熱抽濾，靜置濾液，待沉淀完全，抽濾，沉淀在65℃干燥40min，得到蘆丁的純化物；堿溶酸沉法是用蒸餾水浸泡，加氧化鈣1-1.5g，調(diào)節(jié)PH至8，加熱至微沸，保持40min，趁熱過(guò)濾，浸煮2次，收集濾液，用濃鹽酸調(diào)節(jié)PH至5，靜置至沉淀完全，將沉淀在60-70℃水浴中，溶解，煮沸15min，趁熱抽濾，靜置濾液，待沉淀完全，抽濾，沉淀在

3、65℃干燥40min，得到蘆丁的純化物。本實(shí)驗(yàn)中堿溶酸沉法比超聲法的提取率高。關(guān)鍵詞：槐米；超聲提取法；堿溶酸沉法；紫外分光光度法；聚酰胺薄膜ABSTRACTThisexperimentcomparedultrasonicextractionwithalkalisolutionandacidprecipitationinextractionrateofrutin.UltrasonicmethodissoakedSophorajaponicawith70%ethanol,theultrasonic

4、cleanercarriedouttheSophorajaponicawith30minutes.thenfilteringthesolutionandcarriedouttheresidueintheultrasoniccleaneragain.CollectingthefiltrateinbeakerandAdjustingthefilter’sPHto3withconcentratedhydrochloricacid.Standingthefilterandprecipitatingcom

5、pletely.Collectingtheprecipitatebythefiltration.Theprecipitateiscrudeextractrutin.Recrystallizatingthecrudeextractrutinin60-70℃waterbathandboilingtherecrystallizate’ssolutiononelectricfurnacewith15minutes.Filtratingthesolutionwhenitisboilingandthefil

6、trateasideuntiltheprecipitatecompletely.Filtratingthesolution,Thencollectingtheprecipitate.Theprecipitatedriedat65℃inVacuumovenwith40minutes.Gettingthepurifiedrutin.AlkalisolutionandacidprecipitationissoakedSophorajaponicawithdistilledwater,Addingcal

7、ciumoxide1.5g.adjustingthePHto8,BoilingtheSolutionwith30minutes,thenfilteringthesolutionandcarriedouttheresidueagain,CollectingthefiltrateinbeakerandAdjustingthefilter’sPHto5withconcentratedhydrochloricacid.Standingthefilterandprecipitatingcompletely

8、.Collectingtheprecipitatebythefiltration.Theprecipitateiscrudeextractrutin.Recrystallizatingthecrudeextractrutinin60-70℃waterbathandboilingtherecrystallizate’ssolutiononelectricfurnacewith15minutes.Filtratingthesolutionwhenitishotandthefiltrateasideu