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1、Chapter9:High-levelexpressionofforeigngenes§1.genesilence§2.expressionsystemsofforeigngene§3.IsolatingandpurifyingtheproductofforeigngenesEvaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposePtyLtd.Evaluationonly.Created
2、withAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposePtyLtd.§1.genesilence(基因沉默)GeneSilencing?外源基因存在于生物體內(nèi)，并未丟失或損傷，但該基因不表達或表達量極低的現(xiàn)象。.Genesilencingcanoccurateithertranscriptionallevel(TGS,TranscriptionalGeneSilencing),orpost-transcriptionalleve
3、l(PTGS,Post-TranscriptionalGeneSilencing).Evaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposePtyLtd.Evaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposePtyLtd.TGS:MethylationIn
4、ducedRepeat-InducedGeneSilencing(RIGC),positioneffect,andsoonPTGS:cosuppression,andsoonEvaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposePtyLtd.Evaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Cop
5、yright2004-2011AsposePtyLtd.MethylationInducedEvaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposePtyLtd.Evaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposePtyLtd.Histone:組蛋白de
6、acetylase去乙?；窫valuationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposePtyLtd.Evaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposePtyLtd.基因表達系統(tǒng)原核生物基因表達系統(tǒng)：如大腸桿菌表達系統(tǒng)、芽孢桿菌表達系統(tǒng)、鏈霉菌表達系統(tǒng)、藍
7、藻表達系統(tǒng)等。真核生物基因表達系統(tǒng)：如酵母表達系統(tǒng)、植物細胞表達系統(tǒng)、昆蟲細胞表達系統(tǒng)、哺乳動物細胞表達系統(tǒng)等?！?.expressionsystemsofforeigngeneEvaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposePtyLtd.Evaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.C
8、opyright2004-2011AsposePtyLtd.Evaluationonly.CreatedwithAspose.Slidesfor.NET3.5ClientProfile5.2.0.0.Copyright2004-2011AsposePtyLtd.Evaluationonly.CreatedwithAspose.Slidesfor
