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《dads對(duì)人小細(xì)胞肺癌nci-h446細(xì)胞系增殖抑制的研究》由會(huì)員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫(kù)。
1、·1460·現(xiàn)代生物醫(yī)學(xué)進(jìn)展www．shengwuyixue．comProgressinModernBiomedicineVoL14No．8MAR．2014doi：10．13241／j．cnki．pmb．2014．08．016DADS對(duì)人小細(xì)胞肺癌NCI．H446細(xì)胞系增殖抑制的研究劉德勇張慶麗王海倫彭和人謝海龍△(1湖南環(huán)境職業(yè)技術(shù)學(xué)院病理教研室湖南衡陽(yáng)421005；2南華大學(xué)腫瘤研究所湖南衡陽(yáng)421001)摘要目的：研究二烯丙基二硫(diallyldisulfide，DADS)對(duì)人小細(xì)胞肺癌NCI—H446細(xì)胞增殖的抑制作用，并探討其作用機(jī)制。方法：體外培養(yǎng)NCI．H446細(xì)胞，采用
2、MTT、細(xì)胞計(jì)數(shù)實(shí)驗(yàn)方法檢測(cè)DADS抑制NCI—H446細(xì)胞增殖；通過(guò)HE染色和AO—EB熒光染色方法，觀察DADS處理后NCI—H446細(xì)胞的形態(tài)學(xué)改變。結(jié)果：MTT結(jié)果顯示：DADS作用于NCI—H446細(xì)胞48h后，代謝MTT的能力明顯降低，顯示出較強(qiáng)的細(xì)胞毒性反應(yīng)，Ic5o值介于20~40txg／ml之間。細(xì)胞計(jì)數(shù)結(jié)果表明：DADS作用于NCI—H446細(xì)胞后，隨DADS濃度增加NCI—H446細(xì)胞倍增時(shí)間延長(zhǎng)。HE染色顯示：NCI—H446細(xì)胞經(jīng)DADS處理24h后，與對(duì)照組相比，細(xì)胞體積變小，胞漿豐富，細(xì)胞核變小，染色變淡。AO．EB熒光染色顯示：NCI．H446細(xì)胞經(jīng)DAD
3、S處理24h后，與對(duì)照組相比，細(xì)胞皺縮、呈圓形，胞質(zhì)黃色或橘紅色，細(xì)胞核或細(xì)胞質(zhì)內(nèi)可見(jiàn)致密濃染的黃綠色或橘紅色熒光，并可見(jiàn)橘紅色碎片且隨DADS濃度增加，隨DADS濃度增加細(xì)胞密度逐漸減少。結(jié)論：DADS能抑制體外培養(yǎng)的NCI—H446細(xì)胞增殖，作用效果與藥物濃度及作用時(shí)間相關(guān)。關(guān)鍵詞：二烯丙基二硫；小細(xì)胞肺癌；MTT中圖分類號(hào)：R734．2文獻(xiàn)標(biāo)識(shí)碼：A文章編號(hào)：1673—6273(2014)08-1460-04ProliferationInhibitedinNCI—H440CellswithDADS：IcL1UDe-yong~,ZHANGQing-f,WANGHai-lun1,PEN
4、GHo-ref,XIEHa~lon(1InstituteofPathology,hunanpolytechnicofenvironmentandbiology,Hengyang,Hunan,421005,China；2InstituteofCancerResearch,UniversityofSouthChina,Hengyang,nuBan,421001，China)ABSRACTObjective：ToinvestigatetheefectandthemechanismofDADSonthegrowthofhmnansmal1eel1lungcancerNCI—H446cellsin
5、vitro．Methods：NCI．H446celllineswereculturedinvitro．CellproliferationinhibitionwasmeasuredbyMTTassayandcellcountexperiments．CellmorphologicchangewasobservedbyHEstainingandAO-EBfluorescencestaining．Result：MTTassayshowedthattheabilityofthemetabolicMTTreducedsignificantlyandithadas~ongcytotoxicrespon
6、seaftercultured48hoursbyDADS．IC∞valueslocatedbetween20～40izg／m1．CellcountexperimentsshowedthatNCI-H446cellsaveragedoublingtimeextendedafterexposuretodiferentconcentrationDADS．HEstainingshowedthatcellvolumesandnucleusbecomesmallerwhilecytoplasmbecameabundantandstainhard24hoursafterH446eellularexpo
7、suretoDADS．AO—EBfluorescencestainingshowedthatthecellsmorphoousandcolourhadhugechangedthatdealedwithDADS．Conclusion：DADScouldmarkedlyinhibittheproliferationofNCI—H446andtheefectdependedontheconcentrationandthetimeofDAD
