資源描述:
《不同濃度臭氧對大鼠離體腦片氧糖剝奪及再灌注損傷模型的保護作用.pdf》由會員上傳分享,免費在線閱讀,更多相關(guān)內(nèi)容在應(yīng)用文檔-天天文庫。
1、中國醫(yī)科大學(xué)學(xué)報第43卷第9期2014年9月JournalofChinaMedicalUniversityVo1.43No.9Sep.2014·769··論著·不同濃度臭氧對大鼠離體腦片氧糖剝奪及再灌注損傷模型的保護作用師存?zhèn)ィ磿赠i,冶占福,宋濤(1.青海大學(xué)附屬醫(yī)院疼痛科,西寧810001;2.中國醫(yī)科大學(xué)附屬一院疼痛科,沈陽110001)摘要目的探討不同濃度臭氧對大鼠離體腦片氧糖剝奪及再灌注損傷模型的保護作用。方法制備SD大鼠腦片,室溫下保存在人工腦脊液(ACSF)中,根據(jù)實驗分組情況選擇性經(jīng)過下列程序:恢復(fù)期60min、平衡期30min、氧糖剝奪(OGD)期30min
2、及再灌注期90min。對照組(CTRL)不經(jīng)過OGD期;模型組不施加臭氧干預(yù)(OGD/R);實驗組于再灌注期分別給予10,20,30,40,50p~g/mL的臭氧,并且根據(jù)ACSF中是否含有人血白蛋白(HSA)分成2個亞組。再灌注期結(jié)束后分別測定各組ACSF中谷氨酸(Glu)和乳酸脫氫酶(LDH)的含量。結(jié)果OGD30min后給予90min的再灌注,模型組與對照組比較Glu和LDH釋放增加(P<0.01)。不同濃度的臭氧在不同程度上拮抗了Glu和LDH的釋放,其中40g/mL的臭氧效應(yīng)最明顯,低濃度(10~3Op~g/mL)或高濃度(50p~g/mL)臭氧組與OGD/R組比較
3、效應(yīng)不明顯(P>O.05);在含有HAS組與不合HAS組比較,給予臭氧(40g/mL)干預(yù)能明顯減少Glu和LDH的釋放(P<0.05)。結(jié)論40txg/mL臭氧氣體對大鼠離體腦缺血再灌注損傷模型中的神經(jīng)細(xì)胞具有保護作用,并且HSA能增強臭氧的保護作用。關(guān)鍵詞臭氧;腦缺血;氧糖剝奪中圖分類號R361.3文獻(xiàn)標(biāo)志碼A文章編號0258—4646(2014)O9—0769—04網(wǎng)絡(luò)出版地址http://www.cnki.net/kcms/detail/21.1227.R.20140925.1350.001.htmlNeuro—protectiveEfectofDiferentCon
4、centrationofOzoneonCulturedRatBrainSlicesUnderwentOxygen—glucoseDeprivationandReperfusionSHICun-wei,JINGXiao-peng,YEZhan—fu,SONGTao2(1.DepartmentofPfinManagement,TheAfiliatedHospital,QinghaiUniversity,Xining810001,China;2.Department0fPainManagement,TheFirstHospital,ChiHaMedicalUniversity,Sh
5、enyang110001,China)Abstract0bjectiveToevaluatetheneuro—protectiveefectofdiferentconcentrationofozoneonculturedratbrainsliceswhichweresubjectedtooxygen—glucosedeprivationandreperfusion.MethodsInthisstudy,brainslicesofSDratswereculturedandsubjectedtooxygen—uc0sedepri—vationandrepefusion.Slice
6、sweremaintainedinACSFfor1houratroomtemperature(Recoveryperiod)andthenequilibratedforanadditionalpe-riodof30minutes(Equilibration).Afterwards,thephaseofoxygen/glucosedeprivation(OGD)wascarriedoutfor30minutes.AftertheOGDperi—od,isehemicsolutionwasreplacedby2mLoffresh,oxygenatedACSFfornlladdit
7、ional90一minuteperiod(Reperfusion).SliceswereincubatedinACSFfor120minutes(controlconditions,CTRL)orsubjectedtooxygen/glucosedeprivationfor30minutesfolowedby90一minuteimmersioninnormallyoxygenatedACSF(OGD/R).Increasingconcentrationsofozone(10—50咖L)wereadmin