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1、正交設(shè)計法優(yōu)選六月青多糖膠囊提取工藝葛文漪，黃建春，陳兆霓，焦陽，張士軍，黃仁彬*(廣西醫(yī)科大學(xué)藥理學(xué)教研室，南寧530021)[摘要]目的：優(yōu)選六月青多糖膠囊提取工藝的最佳條件。方法：選用L9(34)正交試驗設(shè)計方法，分別對水煎煮及醇沉兩個步驟進(jìn)行優(yōu)選，以多糖含量為水煎煮工藝評價指標(biāo)；采用苯酚-濃硫酸法測定總多糖含量，以多糖含量和收率為醇沉工藝綜合評價指標(biāo)，確定最佳提取工藝參數(shù)。結(jié)果：六月青多糖膠囊最佳提取工藝為生藥材加10倍量水，每次煎煮2h，煎煮3次。水提液過濾、合并、濃縮至含生藥0.5g·mL-1，7倍量95%乙醇沉
2、淀12h，沉淀1次。結(jié)論：優(yōu)選出的工藝條件,經(jīng)驗證其結(jié)果穩(wěn)定、合理，可作為六月青多糖膠囊的最佳提取工藝。[關(guān)鍵詞]六月青膠囊；正交試驗；提取工藝；煎煮法；醇沉法OptimizationofExtractionTechnologyforLiuyueqingPolysaccharidesCapsulebyOrthogonalDesignGEWen-yi,HUANGJian-chun,CHENZhao-ni,JIAOYang,ZHANGShi-jun,,HUANGRen-bin(DepartmentofPharmacology,G
3、uangxiMedicalUniversity,Nanning530021,China)[Abstract]Objective:TooptimizethetechnologyofextractionforLiuyueqingpolysaccharidescapsules.Method:ThetechnicalconditionsforthewaterdecoctionandthealcoholprecipitationwereoptimizedrespectivelybyorthogonalexperimentdesignL
4、9(34).Thecontentofpolysaccharideswasusedastheindexforthewaterdecoction,thecontentofpolysaccharidesandextractratewereusedasindexesforalcoholprecipitation.Themethodofphenol-sulfuricacidreactionwasusedindeterminingthecontentofpolysaccharides.Optimalprocesscontrolparam
5、eterswere______________________________[收稿日期][基金項目]廣西科學(xué)研究與技術(shù)開發(fā)計劃項目(桂科攻10124008-6，桂科攻0992003A-2，KFJJ2010-22)[第一作者]葛文漪，碩士研究生，從事藥物質(zhì)量控制和生化藥理學(xué)研究，Tel:15994443433,E-mail:gewenyi1986@163.com[通訊作者]*黃仁彬，教授，博士研究生導(dǎo)師，從事抗病毒性肝炎藥物和生化藥理學(xué)研究，Tel:0771-5339805,E-mail:huangrenbin518@163
6、.comdetermined.Result:TheoptimalconditionsforLiuyueqingpolysaccharidescapsuleswereasfollows:decoctingthecrudedrugsthreetimeswith10-foldwater,2hourseachtime.Thephysicliquorextractedbywaterwasfiltered,mixedandconcentratedto0.5g·mLl-1(crudedrug),andthenprecipitatedby7
7、-fold95%alcoholonetimefor12hours.Conclusion:Theresultofthisoptimizedextractionprocedureisprovedtobestableandreliable,itcanbeusedastheoptimalextractionprocedureofLiuyueqingpolysaccharidescapsules.[Keywords]Liuyueqingpolysaccharidescapsules,orthogonalexperimentdesign
8、,extractiontechnology,decoctionmethod,alcohol-precipitationmethod六月青為廣西特色民間草藥，系爵床科(Acanthaceae)肖雞籠屬植物肖雞籠（頂花馬蘭）Taraphochlamysaffinis(Giff)Bremekhu
