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《根癌農(nóng)桿菌介導(dǎo)的ANTICTLA4HS83基因遺傳轉(zhuǎn)化甘薯的研究》由會員上傳分享，免費(fèi)在線閱讀，更多相關(guān)內(nèi)容在學(xué)術(shù)論文-天天文庫。
1、西南交通大學(xué)碩士學(xué)位論文根癌農(nóng)桿菌介導(dǎo)的ANTI-CTLA-4-HS83基因遺傳轉(zhuǎn)化甘薯的研究姓名：許培芳申請學(xué)位級別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：李萍;閻文昭20080401西南交通大學(xué)碩士研究生學(xué)位論文第1l頁AbstractPlant-basedexpressionofpharmaceuticalproteinsisacOSt—effectivealtern．a(chǎn)tivetotraditionallarge—scaleproductionsystemssuchasmicrobialferment
2、ation，insectandmammaliancellcultures，andtransgenicanimals．Plantsarethemosteconomicalmakersofbiomassusingonlysunlight，water，carbondioxideandminerals．Theyofferapropermodificationsystemforeukaryoticproteins，suchasfolding，post-tr．a(chǎn)nslationalmodification，andsub—c
3、ellulartargeting；moreover，theyavoidanimalcell—culturecontaminantssuchasmammalianpmhogensandextraneousviralorbacterialcomponents．Sincethehumangrowthhormonewasexpressedintransgenictobaccoin1986，manymorepharmaceuticallyrelevantproteinshavebeensuccessfullyproduc
4、edinplants．TheproteinsexpressedinplantsCanbeextractedfrom．wholeplantpartsororganellesandusedasasourceformedicinalapplications．Productionofsweetpotato(Ipomoeabatatas(L)Lava．)ranksfifthafterthatofrice，wheat，maizeandwhitepotato，amongthefoodcropsoftheworld．Sweet
5、potatoisoneofthemostimportantcropsfordevelopingcountries，whereitrankssecondaccordingtoitsproductionvalue，andfifthaccordingtoitscaloriecontri--butionforhumans．Sweetpotatohaveahighproductionyieldofbiomass，needlessnutrientsfromthesoilthanmostothercrops．Establis
6、hingsweetpotatoasaproductionsiteforthedeliveryofrecOmbinantproteinsisworthstrivingfor．Singlechainfragmentvariables(scFv)anti—CTLA-hs83isonekindoftargetfragmentsforimmunotoxins，itCallselectivelylinktheactivatedTcells，possessesthefeaturesofspecificity，highacii
7、vityandlowimmunogenicity．Soitmaybehavegoodprospectforpreventionandcureoftransplantrejection．西南交通大學(xué)碩士研究生學(xué)位論文第1II頁Inthisstudy，wedescribeallagrobacteriumbinaryvectorcarryingsinglechainvariablefragment(scFv)anti··CqT．A·-hs83genewassuccessfullyconstructedbasedonp
8、CAMBIAl301，thentransformedsweetpotatoplantswiththisbinaryvectorusingA．tumefaciens-mediatedtransformation，30hygromycinresistantplantswereobtained．Hygromycinresistantplantsweretestedbypolymerasech
